Frontiers in Plant Science (Apr 2016)

A High-Density Genetic Map for Cucumber (Cucumis Sativus L.) Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber

  • Wenying eZhu,
  • Long eHuang,
  • Long eChen,
  • Jiantao eYang,
  • Jiani eWu,
  • Meiling eQu,
  • Danqing eYao,
  • Chunli eGuo,
  • Hongli eLian,
  • Huanle eHe,
  • Junsong ePan,
  • Run eCai

DOI
https://doi.org/10.3389/fpls.2016.00437
Journal volume & issue
Vol. 7

Abstract

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High-density genetic linkage map plays an important role in genome assembly and QTL fine mapping. Since the coming of next-generation sequencing (NGS), makes the structure of high-density linkage maps much more convenient and practical, which simplifies SNP discovery and high-throughput genotyping. In this research, a high-density linkage map of cucumber was structured using specific length amplified fragment sequencing, using 153 F2 populations of S1000×S1002. The high-density genetic map composed 3,057 SLAFs, including 4,475 SNP markers on 7 chromosomes, and spanned 1061.19cM. The average genetic distance is 0.35cM. Based on this high-density genome map, QTL analysis was performed on two cucumber fruit traits, fruit length and fruit diameter. There are 15 QTLs for the two fruit traits were detected.

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