Sladek Z, Rysanek D: CD14 expression, apoptosis and necrosis in resident and inflammatory macrophages from virgin bovine mammary gland

Abstract

This paper investigates the association between expression of CD14 and occurrence of apoptosis in blood monocytes, resident (RESMAC) and inflammatory macrophages from heifer mammary glands after infusion of PBS (INFMACPBS) or LPS (INFMACLPS). Resident macrophages (RESMAC) were obtained before, and inflammatory macrophages (INFMACPBS and INFMACLPS) 24 h after, induction of an inflammatory response using phosphate buffered saline (PBS) and lipopolysaccharide (LPS) in mammary glands of unbred heifers. Cell samples were analysed for differential counts, CD14 expression, apoptosis and necrosis using flow cytometry. In vitro cultivation led to a decrease in the proportion of living cells and to an increase in the proportion of apoptotic and necrotic cells in all macrophages and blood monocytes. In CD14+ macrophages, the proportions of live cells increased and proportions of apoptotic and necrotic cells decreased after in vitro cultivation. We observed in CD14- macrophages and monocytes that the proportions of live cells decreased and proportions of apoptotic and necrotic cells increased after in vitro cultivation. Our experiments confirm that the expression of CD14 in bovine mammary gland macrophages and blood monocytes is associated with cell viability.

Keywords

• virgin bovine mammary gland
• in vitro
• macrophages
• cd14
• apoptosis
• cytokines
• lipopolysaccharide