Arquivo Brasileiro de Medicina Veterinária e Zootecnia ()

Phospholipid composition and resistance to vitrification of in vivo blastocyst of a Brazilian naturalized porcine breed

  • J.F.W. Sprícigo,
  • L.O. Leme,
  • A.L. Guimarães,
  • J.C. Oliveira Neto,
  • P.C.P. Silva,
  • N.H. Moreira,
  • I. Pivato,
  • B.D.M. Silva,
  • A.F. Ramos,
  • M.A.N. Dode

DOI
https://doi.org/10.1590/1678-4162-10249
Journal volume & issue
Vol. 71, no. 3
pp. 837 – 847

Abstract

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ABSTRACT Piau porcine blastocysts were submitted to MALDI-TOF to identify the main phospholipids (PL). After that, in vivo blastocysts (D6) were vitrified (n=52), non-vitrified were used as control (n=42). After warming, blastocysts were in vitro cultured to assess re-expansion and hatching at 24 and 48 hours. Finally, at 48 hours, hatched blastocysts were submitted to RT-qPCR searching for BCL2A1, BAK, BAX and CASP3 genes. For MALDI-TOF, the ion intensity was expressed in arbitrary units. Blastocyst development was compared by Qui-square (P 0.05) between fresh or vitrified blastocysts at 24 (33.3%; 32.7%) or 48 hours (2.4%; 13.5%). Hatching rates were higher (P< 0.05) for fresh compared to vitrified at 24 (66.7%; 15.4%) and 48 hours (97.6%; 36.0%). BAX was overexpressed (P< 0.05) after vitrification. In conclusion, Piau blastocysts can be cryopreserved by Cryotop. This study also demonstrated that the apoptotic pathway may be responsible for the low efficiency of porcine embryo cryopreservation.

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