Scientific Reports (Mar 2024)
Diving into the proteomic atlas of SARS-CoV-2 infected cells
- Victor C. Carregari,
- Guilherme Reis-de-Oliveira,
- Fernanda Crunfli,
- Bradley J. Smith,
- Gabriela Fabiano de Souza,
- Stéfanie Primon Muraro,
- Veronica M. Saia-Cereda,
- Pedro H. Vendramini,
- Paulo A. Baldasso,
- Lícia C. Silva-Costa,
- Giuliana S. Zuccoli,
- Caroline Brandão-Teles,
- André Antunes,
- Aline F. Valença,
- Gustavo G. Davanzo,
- João Victor Virgillio-da-Silva,
- Thiago dos Reis Araújo,
- Raphael Campos Guimarães,
- Felipe David Mendonça Chaim,
- Elinton Adami Chaim,
- Carolina Mie Kawagosi Onodera,
- Raissa Guimarães Ludwig,
- Tatiana Dandolini Saccon,
- André R. L. Damásio,
- Luiz Osório S. Leiria,
- Marco Aurélio R. Vinolo,
- Alessandro S. Farias,
- Pedro M. Moraes-Vieira,
- Marcelo A. Mori,
- José Luiz P. Módena,
- Daniel Martins-de-Souza
Affiliations
- Victor C. Carregari
- Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- Guilherme Reis-de-Oliveira
- Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- Fernanda Crunfli
- Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- Bradley J. Smith
- Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- Gabriela Fabiano de Souza
- Laboratory of Emerging Viruses, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP)
- Stéfanie Primon Muraro
- Laboratory of Emerging Viruses, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP)
- Veronica M. Saia-Cereda
- Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- Pedro H. Vendramini
- Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- Paulo A. Baldasso
- Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- Lícia C. Silva-Costa
- Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- Giuliana S. Zuccoli
- Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- Caroline Brandão-Teles
- Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- André Antunes
- Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- Aline F. Valença
- Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- Gustavo G. Davanzo
- Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP)
- João Victor Virgillio-da-Silva
- Department of Pharmacology, Ribeirão Preto Medical School (FMRP), University of São Paulo (USP)
- Thiago dos Reis Araújo
- Obesity and Comorbidities Research Center (OCRC)
- Raphael Campos Guimarães
- Center for Research in Inflammatory Diseases
- Felipe David Mendonça Chaim
- Department of Surgery, Faculty of Medical Sciences, University of Campinas
- Elinton Adami Chaim
- Department of Surgery, Faculty of Medical Sciences, University of Campinas
- Carolina Mie Kawagosi Onodera
- Hematology-Hemotherapy Center, University of Campinas
- Raissa Guimarães Ludwig
- Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- Tatiana Dandolini Saccon
- Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- André R. L. Damásio
- Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- Luiz Osório S. Leiria
- Department of Pharmacology, Ribeirão Preto Medical School (FMRP), University of São Paulo (USP)
- Marco Aurélio R. Vinolo
- Obesity and Comorbidities Research Center (OCRC)
- Alessandro S. Farias
- Instituto Nacional de Biomarcadores em Neuropsiquiatria (INBION), Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Pedro M. Moraes-Vieira
- Laboratory of Immunometabolism, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP)
- Marcelo A. Mori
- Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- José Luiz P. Módena
- Laboratory of Emerging Viruses, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas (UNICAMP)
- Daniel Martins-de-Souza
- Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP)
- DOI
- https://doi.org/10.1038/s41598-024-56328-3
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 14
Abstract
Abstract The COVID-19 pandemic was initiated by the rapid spread of a SARS-CoV-2 strain. Though mainly classified as a respiratory disease, SARS-CoV-2 infects multiple tissues throughout the human body, leading to a wide range of symptoms in patients. To better understand how SARS-CoV-2 affects the proteome from cells with different ontologies, this work generated an infectome atlas of 9 cell models, including cells from brain, blood, digestive system, and adipocyte tissue. Our data shows that SARS-CoV-2 infection mainly trigger dysregulations on proteins related to cellular structure and energy metabolism. Despite these pivotal processes, heterogeneity of infection was also observed, highlighting many proteins and pathways uniquely dysregulated in one cell type or ontological group. These data have been made searchable online via a tool that will permit future submissions of proteomic data ( https://reisdeoliveira.shinyapps.io/Infectome_App/ ) to enrich and expand this knowledgebase.
