Supplementation of molecular data and studying the cultural characteristics of Sapa shiitake mushroom Lentinula edodes

Abstract

Samples of Sapa shiitake mushroom (named Len026), growing on decaying broadleaf stems, were collected in the mountainous areas of Son Binh commune, Tam Duong district, Lao Cai province at the end of May 2019. External morphological features: the mushroom cap has fibrous scales on its surface, reddish brown (when being wet) turn into yellow brown, with slight cracks (when being dry); thin cap flesh; in young fruiting bodies, rolled cap edge, flattened to slightly raised in mature ones. Microscopic features the elliptic structure of the spores and especially the existence of pleurocystidia and cheilocystidia. The above features indicate that this sample has quite a mix of all three characteristics species Lentinula edodes, Lentinula lateritia and Lentinula boryana, which have been identified that may exist in our country. However, the ITS rDNA analysis data for this mushroom shows that this is a species of Lentinula edodes. Purebred of this strain has been isolated from fruiting body tissue. In the conditions of artificial cultivation on the rubber wood sawdust substrate, the mushroom grew and developed very well. Although the biological efficiency of strain Len026 was still quite low, the characteristics such as: rapid growth of mycelium and browning of mycelium as well as the ability to form very strong fungal sprouts indicate that this strain is potential to be used as a raw material for breeding new mushroom varieties for local technology of growing shiitake mushrooms.

Keywords

• hình thái giải phẫu
• itsrdna
• giống nấm
• lentinula edodes
• sapa