Frontiers in Microbiology (Dec 2022)
Antifungal susceptibility profiles and drug resistance mechanisms of clinical Candida duobushaemulonii isolates from China
- Xin-Fei Chen,
- Xin-Fei Chen,
- Xin-Fei Chen,
- Han Zhang,
- Han Zhang,
- Xin-Miao Jia,
- Xin-Miao Jia,
- Jin Cao,
- Li Li,
- Xin-Lan Hu,
- Ning Li,
- Yu-Ling Xiao,
- Fei Xia,
- Li-Yan Ye,
- Qing-Feng Hu,
- Xiao-Li Wu,
- Li-Ping Ning,
- Po-Ren Hsueh,
- Po-Ren Hsueh,
- Xin Fan,
- Shu-Ying Yu,
- Shu-Ying Yu,
- Jing-Jing Huang,
- Jing-Jing Huang,
- Jing-Jing Huang,
- Xiu-Li Xie,
- Xiu-Li Xie,
- Wen-Hang Yang,
- Wen-Hang Yang,
- Wen-Hang Yang,
- Ying-Xing Li,
- Ying-Xing Li,
- Ge Zhang,
- Ge Zhang,
- Jing-Jia Zhang,
- Jing-Jia Zhang,
- Si-Meng Duan,
- Si-Meng Duan,
- Wei Kang,
- Wei Kang,
- Tong Wang,
- Tong Wang,
- Jin Li,
- Jin Li,
- Meng Xiao,
- Meng Xiao,
- Xin Hou,
- Ying-Chun Xu,
- Ying-Chun Xu
Affiliations
- Xin-Fei Chen
- Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Xin-Fei Chen
- Graduate School, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Xin-Fei Chen
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Han Zhang
- Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Han Zhang
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Xin-Miao Jia
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Xin-Miao Jia
- Medical Research Center, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Jin Cao
- Jinling Hospital Institute of Clinical Laboratory Science, School of Medicine, Nanjing University, Nanjing, Jiangsu, China
- Li Li
- Department of Dermatology, Hua Shan Hospital, Fudan University, Shanghai, China
- Xin-Lan Hu
- Department of Laboratory Medicine, Fujian Provincial Hospital, Fuzhou, China
- Ning Li
- Department of Laboratory Medicine, Fujian Provincial Hospital, Fuzhou, China
- Yu-Ling Xiao
- Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China
- Fei Xia
- Department of Laboratory Medicine, Ruian People's Hospital, Wenzhou, China
- Li-Yan Ye
- 0Department of Laboratory Medicine, The First Medicine Center, Chinese PLA General Hospital, Beijing, China
- Qing-Feng Hu
- 1Department of Laboratory Medicine, Zhejiang Provincial People's Hospital, Hangzhou, China
- Xiao-Li Wu
- 2Department of Laboratory Medicine, The People’s Hospital of Liaoning Province, Shenyang, China
- Li-Ping Ning
- 3Department of Laboratory Medicine, No.908 Hospital of Joint Logistics Support Force, Nanchang, China
- Po-Ren Hsueh
- 4Department of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan
- Po-Ren Hsueh
- 5Department of Laboratory Medicine and Internal Medicine, China Medical University Hospital, China Medical University, Taichung, Taiwan
- Xin Fan
- 6Department of Infectious Diseases and Clinical Microbiology, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China
- Shu-Ying Yu
- Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Shu-Ying Yu
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Jing-Jing Huang
- Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Jing-Jing Huang
- Graduate School, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Jing-Jing Huang
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Xiu-Li Xie
- Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Xiu-Li Xie
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Wen-Hang Yang
- Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Wen-Hang Yang
- Graduate School, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Wen-Hang Yang
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Ying-Xing Li
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Ying-Xing Li
- Medical Research Center, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Ge Zhang
- Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Ge Zhang
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Jing-Jia Zhang
- Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Jing-Jia Zhang
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Si-Meng Duan
- Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Si-Meng Duan
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Wei Kang
- Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Wei Kang
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Tong Wang
- Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Tong Wang
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Jin Li
- Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Jin Li
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Meng Xiao
- Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Meng Xiao
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- Xin Hou
- 7Department of Laboratory Medicine, Peking University Third Hospital, Beijing, China
- Ying-Chun Xu
- Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China
- Ying-Chun Xu
- Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China
- DOI
- https://doi.org/10.3389/fmicb.2022.1001845
- Journal volume & issue
-
Vol. 13
Abstract
Candida duobushaemulonii, type II Candida haemulonii complex, is closely related to Candida auris and capable of causing invasive and non-invasive infections in humans. Eleven strains of C. duobushaemulonii were collected from China Hospital Invasive Fungal Surveillance Net (CHIF-NET) and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF), VITEK 2 Yeast Identification Card (YST), and internal transcribed spacer (ITS) sequencing. Whole genome sequencing of C. duobushaemulonii was done to determine their genotypes. Furthermore, C. duobushaemulonii strains were tested by Sensititre YeastOne™ and Clinical and Laboratory Institute (CLSI) broth microdilution panel for antifungal susceptibility. Three C. duobushaemulonii could not be identified by VITEK 2. All 11 isolates had high minimum inhibitory concentrations (MICs) to amphotericin B more than 2 μg/ml. One isolate showed a high MIC value of ≥64 μg/ml to 5-flucytosine. All isolates were wild type (WT) for triazoles and echinocandins. FUR1 variation may result in C. duobushaemulonii with high MIC to 5-flucytosine. Candida duobushaemulonii mainly infects patients with weakened immunity, and the amphotericin B resistance of these isolates might represent a challenge to clinical treatment.
Keywords
- Candida duobushaemulonii
- antifungal susceptibility
- FUR1
- whole genome sequence
- drug resistance mechanisms
