Establishment of two induced pluripotent stem cell line from healthy elderly (IPTi005-A and IPTi007-A)

Lin Zhang; Qiao Ren; Gang Liu; Rongrong Wu; Shulin Meng; Kang Xiahou; Ning Jiang; Yongxiang Zhang; Wenxia Zhou

Stem Cell Research (May 2020)

Establishment of two induced pluripotent stem cell line from healthy elderly (IPTi005-A and IPTi007-A)

Lin Zhang,
Qiao Ren,
Gang Liu,
Rongrong Wu,
Shulin Meng,
Kang Xiahou,
Ning Jiang,
Yongxiang Zhang,
Wenxia Zhou

Affiliations

Lin Zhang: Beijing Institute of Pharmacology and Toxicology; Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures; Beijing, China; School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University; Shenyang, China
Qiao Ren: Beijing Institute of Pharmacology and Toxicology; Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures; Beijing, China
Gang Liu: Beijing Institute of Pharmacology and Toxicology; Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures; Beijing, China
Rongrong Wu: Beijing 302 Hospital; Beijing, China
Shulin Meng: IxCell BIOTECHNOLOGY Co.,Ltd; Shanghai, China
Kang Xiahou: IxCell BIOTECHNOLOGY Co.,Ltd; Shanghai, China
Ning Jiang: Beijing Institute of Pharmacology and Toxicology; Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures; Beijing, China; Corresponding author.
Yongxiang Zhang: Beijing Institute of Pharmacology and Toxicology; Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures; Beijing, China; School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University; Shenyang, China; Corresponding author.
Wenxia Zhou: Beijing Institute of Pharmacology and Toxicology; Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures; Beijing, China; Corresponding author.

Journal volume & issue: Vol. 45

Abstract

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Two healthy elderly donated their peripheral blood mononuclear cells (PBMCs). The induced pluripotent stem cell lines (IPTi005-A and IPTi007-A) were reprogrammed by episomal vector system with Yamanaka factors OCT4, SOX2, C-MYC and KLF4. Both the two iPSC lines can differentiate into three germ layers determined by embryoid bodies (EBs) differentiation. Flow cytometry analysis showed that more than 90% cells expressed NANOG, OCT4 and SSEA4. Besides, the iPSC lines of IPTi005-A and IPTi007-A were confirmed to exhibit a normal karyotype. In the studies of age-related disease, the iPSC lines can be used as controls.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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