A novel self-enhanced electrochemiluminescent aptamer sensor based on ternary nanocomposite PEI/RuSi-MWCNTs for the detection of profenofos residues in vegetables
Zhenying He,
Haifang Wang,
Wenzheng Liu,
Jiashuai Sun,
Jingcheng Huang,
Jie Han,
Baoxin Li,
Rui Xu,
Yuhao Zhang,
Jin Hua,
Yemin Guo,
Fangyuan Lu,
Ce Shi
Affiliations
Zhenying He
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Information Technology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China
Haifang Wang
Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, China
Wenzheng Liu
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China
Jiashuai Sun
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China
Jingcheng Huang
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China
Jie Han
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China
Baoxin Li
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China
Rui Xu
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China
Yuhao Zhang
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China
Jin Hua
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China
Yemin Guo
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China
Fangyuan Lu
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Zibo City Key Laboratory of Agricultural Product Safety Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China; Corresponding author. School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 266 Xincun Xilu, Zibo, Shandong, 255049, China.
Ce Shi
Information Technology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China; Corresponding author. Information Technology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
In this work, a novel ternary nanocomposite of PEI/RuSi-MWCNTs was designed and synthesized for the first time, which an ultrasensitive and self-enhanced electrochemiluminescent (ECL) aptasensor was developed for the detection of profenofos residues in vegetables. The self-enhanced complex PEI-Ru (II) enhanced the emission and stability of ECL, and the multi-walled carbon nanotubes (MWCNTs) acted as an excellent carrier and signal amplification. The PEI/RuSi-MWCNTs were characterized by scanning electron microscope (SEM), transmission electron microscope (TEM) and energy dispersive spectrometer (EDS). The incorporation of gold nanoparticles (AuNPs) improved the performance of the sensor and provided a platform for the immobilization of the aptamer. The results of the experiment showed that the presence of profenofos significantly suppressed the electrochemiluminescence intensity of the sensor. The detection sensitivity of the aptamer sensor was in the range of 1 × 10−2 to 1 × 103 ng/mL. Under optimal conditions, the limit of detection (LOD) of the sensor for profenofos was 1.482 × 10−3 ng/mL. The sensor had excellent stability, reproducibility and specificity. The recoveries of the sensor ranged from 92.29 % to 106.47 % in real sample tests.
