L-天冬氨酸离子液体联合猪胰脂肪酶 催化油酸酯化工艺Esterification of oleic acid catalyzed by L-Aspartic acid ionic liquid combined with porcine pancreatic lipase

Abstract

为绿色高效制备生物柴油，利用L-天冬氨酸离子液体（\[Asp\]HSO4）联合猪胰脂肪酶催化油酸制备油酸甲酯，采用单因素实验探究了反应时间、醇酸物质的量比、\[Asp\]HSO4用量、猪胰脂肪酶用量和反应温度对转化率的影响，在此基础上，采用正交实验进行优化，得到\[Asp\]HSO4联合猪胰脂肪酶催化油酸酯化反应的最优工艺条件为\[Asp\]HSO4用量6%、醇酸物质的量比5.5∶ 1、猪胰脂肪酶用量4%、反应时间21 h、反应温度45 ℃，在此条件下转化率可达91.91%。\[Asp\]HSO4可降低甲醇和温度对猪胰脂肪酶催化活性的影响，对猪胰脂肪酶催化油酸酯化具有协同效应。 In order to prepare biodiesel greenly and efficiently，L-Aspartic acid ionic liquid (\[Asp\]HSO4) combined with porcine pancreatic lipase was used to catalyze the esterification of oleic acid to prepare methyl oleate. The effects of reaction time, molar ratio of methanol to oleic acid, amount of \[Asp\]HSO4, amount of porcine pancreatic lipase, and the reaction temperature on the conversion rate were investigated by single factor experiment.Then the esterification process conditions were optimized by orthogonal experiment as follows: amount of \[Asp\]HSO4 6%, molar ratio of methanol to oleic acid 5.5∶ 1, amount of porcine pancreatic lipase 4%, reaction time 21 h, and reaction temperature 45 ℃. The conversion rate could reach 91.91% under the optimal conditions.\[Asp\]HSO4can reduce the effects of methanol and temperature on the catalytic activity of porcine pancreatic lipase,and has synergistic effect on the esterification of oleic acid catalyzed by porcine pancreatic lipase.

Keywords

• 油酸甲酯；天冬氨酸离子液体；脂肪酶；酯化反应
• methyl oleate; l-aspartic acid ionic liquid; lipase;esterification reaction