Platinum Nanoparticles As A Therapeutic Agent Against Dextran Sodium Sulfate-Induced Colitis In Mice

Abstract

Suqin Zhu,1,2 Mingyong Zeng,2 Guangxin Feng,2 Haohao Wu2 1Institute of Nutrition and Health, Qingdao University, Qingdao 266021, People’s Republic of China; 2College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong Province, 266003, People’s Republic of ChinaCorrespondence: Haohao WuCollege of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, Shandong Province 266003, People’s Republic of ChinaTel [phone] Fax [phone] Email [email protected]: This study aimed to evaluate the anti-colitis potential of platinum nanoparticles (PtNPs).Materials and methods: 5-, 30- and 70-nm PtNPs were administered to C57BL/6 mice once daily by intragastric gavage for 8 d during and after 5-d dextran sodium sulfate treatment.Results: According to body weight change, stool blood and consistency, and colon length and histopathology, PtNPs size-dependently alleviated DSS-induced murine colitis. PtNPs enhanced gut-barrier function by upregulating the colonic expressions of heat-shock protein 25 and tight junction proteins. Based on colonic myeloperoxidase activity, colonic and peripheral levels of interleukin-6 and tumor necrosis factor-α, and peripheral counts of white blood cells, PtNPs attenuated colonic and systemic inflammation. By suppressing lipopolysaccharide-triggered production of proinflammatory mediators, including nitric oxide, tumor necrosis factor-α and interleukin-6, PtNPs exerted direct anti-inflammatory activities in RAW264.7 macrophages through a mechanism involving intracellular reactive oxygen species scavenging and Toll-like receptor 4/NF-κB signaling suppression. High-throughput 16S rRNA sequencing of fecal samples unveiled that PtNPs induced gut dysbiosis by unfavorably altering α-diversity, Firmicutes/Bacteroidetes ratio, and richness of certain specific bacteria.Conclusion: PtNPs are a promising anti-colitis agent, but may negatively impact gut-microbiota.Keywords: platinum nanoparticles, ulcerative colitis, gut-microbiota, anti-inflammation, gut-barrier function

Keywords

• platinum nanoparticles
• ulcerative colitis
• gut microbiota
• anti-inflammation
• gut barrier function.