Experimental Study of Immunological Activity of Live Plague Vaccine Subjected to Extreme Temperature

Abstract

The study has resulted in the comparative estimate of the immunological activity of the preparations (LPV) with optimal storage mode and after exposure to extreme temperature (37 ± 1 °С) for 6 days, followed by storage at (4 ± 2 °С). Data obtained during the study indicate an identical immune response. After-heat LPV sample did not reduce the ability to exercise immunological activity during the three-year storage (shelf-life of the drug). When the animals vaccination in this group, total number of T-lymphocytes are increased and signs of humoral immunity are shown earlier. Thus, we can predict more labile conditions on the transportation of live bacterial vaccine without compromising its characteristics.

Keywords

• immunization
• live plague vaccine
• laboratory animals
• microbial cell y. pestis ev
• flow cytometry
• monoclonal antibodies
• subpopulations of lymphocytes
• immunological memory