The feeding behavior of Diaphorina citri monitored by using an electrical penetration graph (DC-EPG) on citrus plants treated with Bacillus cereus and Bacillus velezensis

Abstract

Diaphorina citri, an important pest and insect vector that can transmit the pathogenic bacteria Candidatus Liberibacter asiaticus, causing Huanglongbing disease, is one of many challenges in citrus agriculture. Integrated pest management by utilizing microorganisms is a wise and efficient alternative without damaging the environment. Utilization of Plant Growth Promoting Rhizobacteria (PGPR), such as Bacillus cereus and B. velezensis, is a potential strategy for the biological control of plant diseases or insect vectors. By inducing systemic resistance in plants, PGPR can enhance plant defense against diseases and insect pests while activating molecular and physiological changes in plants. This research aimed to determine the effect of B. cereus and B. velezensis on the plant growth and feeding behavior of D. citri. The height and volume of the plant canopy were observed periodically for 6 months, while the feeding behavior of D. citri was monitored using the Electrical Penetration Graph (DC-EPG). The results showed increased height and volume of the citrus plant canopy treated with B. cereus, indicating that B. cereus could act as a PGPR. The application of B. cereus and B. velezensis to citrus seedlings affected the feeding behavior of D. citri. D citri showed difficulty in penetrating the phloem tissue of citrus plants.

Keywords

• bacillus cereus
• bacillus velezensis
• citrus
• diaphorina citri
• electrical penetrationgraph