A Disposable Saliva Electrochemical MIP-Based Biosensor for Detection of the Stress Biomarker α-Amylase in Point-of-Care Applications

Tânia S. C. R. Rebelo; Inês M. Miranda; Ana T. S. C. Brandão; Laura I. G. Sousa; José A. Ribeiro; António F. Silva; Carlos M. Pereira

doi:10.3390/electrochem2030028

Electrochem (Jul 2021)

A Disposable Saliva Electrochemical MIP-Based Biosensor for Detection of the Stress Biomarker α-Amylase in Point-of-Care Applications

Tânia S. C. R. Rebelo,
Inês M. Miranda,
Ana T. S. C. Brandão,
Laura I. G. Sousa,
José A. Ribeiro,
António F. Silva,
Carlos M. Pereira

Affiliations

Tânia S. C. R. Rebelo: CIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, Portugal
Inês M. Miranda: CIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, Portugal
Ana T. S. C. Brandão: CIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, Portugal
Laura I. G. Sousa: CIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, Portugal
José A. Ribeiro: CIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, Portugal
António F. Silva: CIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, Portugal
Carlos M. Pereira: CIQUP—Chemistry Research Center, Department of Chemistry and Biochemistry Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, s/n, 4169-007 Porto, Portugal

DOI: https://doi.org/10.3390/electrochem2030028
Journal volume & issue: Vol. 2, no. 3
pp. 427 – 438

Abstract

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The design and synthesis of artificial receptors based on molecular imprinting (MI) technology for the development of a new MIP-based biosensor for detection of the stress biomarker α-amylase in human saliva in point-of-care (PoC) applications is described in this work. The portable electrochemical devices for monitoring α-amylase consists of cost-effective and disposable gold screen-printed electrodes (AuSPEs). To build the electrochemical device, the template biomolecule was firstly immobilized directly over the working area of the gold chip previously activated with a self-assembled monolayer (SAM) of cysteamine (CA). Then, pyrrole (Py) monomer was selected as building block of a polymeric network prepared by CV electropolymerization. After the electropolymerization process, the enzyme was removed from the polymer film in order to build the specific recognition sites for the target enzyme. The MIP biosensor showed a very wide linear concentration range (between 3.0 × 10−4 to 0.60 mg mL−1 in buffer solution and between 3.0 × 10−4 to 3.0 × 10−2 mg mL−1 in human saliva) and low detection levels were achieved (LOD −4 mg mL−1) using square wave voltammetry (SWV) as the electroanalytical technique.

Published in Electrochem

ISSN: 2673-3293 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Industrial electrochemistry
Website: https://www.mdpi.com/journal/electrochem

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