Effects of Detraining on Ach, AchE, β-amyloid, Apolipoprotein Expression and Cognitive Function in Brain Tissue of Memory Impairment Rats

Abstract

PURPOSE The purpose of this study was to investigate the effects of detraining on ACh, AChE, β-amyloid, apolipoprotein expression and cognitive function in brain tissue of memory impairment rats. METHODS Sparague-Dawley rats (n=40) were randomly divided into 4 groups (age=7 weeks, n=10 in each group): control group (CON), scopolamine-induced control group (SCO-CON), scopolamine+low intensity exercise group (SCO-LEX), and scopolamine+high intensity exercise group (SCO-HEX). The exercise groups were trained progressively on a treadmill, five days per a week for eight weeks following treadmill running programs: i) low intensity (1-4 weeks: 10 m/min for 30 min, 5-8 weeks: 12 m/min for 30 min), ii) high intensity (1-4 weeks: 15 m/min for 30 min, 5-8 weeks: 16 m/min for 30 min). After 8 weeks-exercise programming, detraining was conducted for the same period. RESULTS After detraining for 8 weeks, both exercise groups significantly increased Ach concentration compared with SCO-CON. Exercise groups significantly reduced AChE activity, β-amyloid and ApoE ε4 protein volumes compared to the SCO-CON. Both exercise groups showed a significant decrease of escape latency compared to the control group. CONCLUSIONS In spite of the effects of detraining, regular exercise is still considered to have a positive influence on the changes of memory impairment related factors.

Keywords

• detraining
• scopolamine
• cognitive function
• memory impairment
• cholinergic