Robust and flexible platform for directed evolution of yeast genetic switches

Masahiro Tominaga; Kenta Nozaki; Daisuke Umeno; Jun Ishii; Akihiko Kondo

doi:10.1038/s41467-021-22134-y

Nature Communications (Mar 2021)

Robust and flexible platform for directed evolution of yeast genetic switches

Masahiro Tominaga,
Kenta Nozaki,
Daisuke Umeno,
Jun Ishii,
Akihiko Kondo

Affiliations

Masahiro Tominaga: Graduate School of Science, Technology and Innovation, Kobe University
Kenta Nozaki: Graduate School of Science, Technology and Innovation, Kobe University
Daisuke Umeno: Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Chiba University
Jun Ishii: Graduate School of Science, Technology and Innovation, Kobe University
Akihiko Kondo: Graduate School of Science, Technology and Innovation, Kobe University

DOI: https://doi.org/10.1038/s41467-021-22134-y
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 13

Abstract

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Eukaryotic genetic switches are more complex than prokaryotic ones, complicating their design. Here the authors present a workflow for parallel screening, selection and evolution of yeast genetic switches.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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