Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
Charisse Petersen
Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
Antonio Serapio-Palacios
Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
Tahereh Bozorgmehr
Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
Rachelle Loo
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
Alina Chalanuchpong
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
Bernard Lo
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada
Kelsey E Huus
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
Weronika Barcik
Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
Meghan B Azad
Children’s Hospital Research Institute of Manitoba and Department of Pediatrics and Child Health, University of Manitoba, WinnipegMB, Canada
Allan B Becker
Children’s Hospital Research Institute of Manitoba and Department of Pediatrics and Child Health, University of Manitoba, WinnipegMB, Canada
Piush J Mandhane
Department of Pediatrics, University of Alberta, Edmonton, Canada; School of Public Health, University of Alberta, Edmonton, Canada
Theo J Moraes
The Hospital for Sick Children, Toronto, Canada
Malcolm R Sears
Department of Medicine, McMaster University, Hamilton, Canada
Padmaja Subbarao
The Hospital for Sick Children, Toronto, Canada; Department of Pediatrics, University of Toronto, Toronto, Canada
Department of Biomedical Engineering, University of British Columbia, Vancouver, Canada; Department of Medical Genetics University of British Columbia, Vancouver, Canada
Stuart E Turvey
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Department of Pediatrics, University of British Columbia, Vancouver, Canada
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada; Michael Smith Laboratories, University of British Columbia, Vancouver, Canada; Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada
Bacterial members of the infant gut microbiota and bacterial-derived short-chain fatty acids (SCFAs) have been shown to be protective against childhood asthma, but a role for the fungal microbiota in asthma etiology remains poorly defined. We recently reported an association between overgrowth of the yeast Pichia kudriavzevii in the gut microbiota of Ecuadorian infants and increased asthma risk. In the present study, we replicated these findings in Canadian infants and investigated a causal association between early life gut fungal dysbiosis and later allergic airway disease (AAD). In a mouse model, we demonstrate that overgrowth of P. kudriavzevii within the neonatal gut exacerbates features of type-2 and -17 inflammation during AAD later in life. We further show that P. kudriavzevii growth and adherence to gut epithelial cells are altered by SCFAs. Collectively, our results underscore the potential for leveraging inter-kingdom interactions when designing putative microbiota-based asthma therapeutics.
