Purification of Staphylococcal Enterotoxin B from local isolates and evaluation of its effect on stomach and small intestine tissue

Abstract

abstractThis study aimed to purify of staphylococcal enterotoxin b (SEB) from clinical samples isolated from different infections including (urine, wound, blood and eye swab) in Iraq. Using ion exchange chromatography (IEX) as primary step followed by gel chromatography for further purification. Ammonium sulfate precipitation used in alteration of acids due to ammonium sulfate most completely meets with the requirements for the salt, which include that it be highly dissolve in water, that its component ions be safe for use with proteins, and that it have a low heat solution,cheapness and commercially available. Results showed there was just one absorption peak (15-38) fractions that represents SEB with the highest activity. After applied it on laboratory animals (Rats) results in strong pathogenicity signs which include lethargy, withdrawal, a clear decline in activity in response to the effect of the toxin and a change in the cornea of the eye, which lead to the death of the first animal after only 48 hours, after the second dose. The protein concentration was 0.2 mg ̸ ml. we conclude The SEB is a highly super antigene that have ability to cause a range of histopathological changes in some body organs ranged from mild to severe depending on dose of SEB, time period and experimental animals, in addition SEB causes death in rare cases.

Keywords

• gel chromatography
• ion exchange chromatography (iex)
• staphylococcal enterotoxin b (seb)
• superantigen