International Journal of Nanomedicine (Mar 2018)
Biomimetic mineralized strontium-doped hydroxyapatite on porous poly(L-lactic acid) scaffolds for bone defect repair
Abstract
Min Ge,1,2 Kun Ge,1,3 Fei Gao,4 Weixiao Yan,1,2 Huifang Liu,2 Li Xue,1 Yi Jin,1,2 Haiyun Ma,1 Jinchao Zhang1,2 1Department of Chemistry, College of Chemistry and Environmental Science, Hebei University, Baoding, People’s Republic of China; 2Key Laboratory of Chemical Biology of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Hebei University, Baoding, People’s Republic of China; 3Department of Science and Technology, Affiliated Hospital of Hebei University, Baoding, People’s Republic of China; 4Department of Orthopedics, Affiliated Hospital of Hebei University, Baoding, People’s Republic of China Introduction: Poly(L-lactic acid) (PLLA) has been approved for clinical use by the US Food and Drug Administration (FDA); however, their stronger hydrophobicity and relatively fast degradation rate restricted their widespread application. In consideration of the composition of bone, the inorganic–organic composite has a great application prospect in bone tissue engineering. Many inorganic–organic composite scaffolds were prepared by directly mixing the active ingredient, but this method is uncontrolled and will lead to lack of homogeneity in the polymer matrix. Strontium (Sr) is an admirable addition to improve the bioactivity and bone induction of hydroxyapatite (HA). To our knowledge, the application of biomimetic mineralized strontium-doped hydroxyapatite on porous poly(L-lactic acid) (Sr-HA/PLLA) scaffolds for bone defect repair has never been reported till date. Biomimetic mineralized Sr-HA/PLLA porous scaffold was developed in this study. The results indicated that the Sr-HA/PLLA porous scaffold could improve the surface hydrophobicity, reduce the acidic environment of the degradation, and enhance the osteoinductivity; moreover, the ability of protein adsorption and the modulus of compression were increased. The results also clearly showed the effectiveness of the Sr-HA/PLLA porous scaffold in promoting cell adhesion, proliferation, and alkaline phosphatase (ALP) activity. The micro computed tomography (micro-CT) results showed that more new bones were formed by Sr-HA/PLLA porous scaffold treatment. The histological results confirmed the osteoinductivity of the Sr-HA/PLLA porous scaffold. The results suggested that the Sr-HA/PLLA porous scaffold has a good application prospect in bone tissue engineering in the future.Purpose: The purpose of this study was to promote the bone repair.Materials and methods: Surgical operation of rabbits was carried out in this study.Results: The results showed that formation of a large number of new bones by the Sr-HA/PLLA porous scaffold treatment is possible.Conclusion: Biomimetic mineralized Sr-HA/PLLA porous scaffold could effectively promote the restoration of bone defects in vivo. Keywords: Sr-HA, PLLA porous scaffold, biomimetic mineralization, bone repair
