Generation of iPS cell lines from schizophrenia patients using a non-integrative method

Jaroslaw Sochacki; Sylvie Devalle; Marcelo Reis; Renata de Moraes Maciel; Bruna da Silveira Paulsen; Helena Brentani; Paulo Silva Belmonte-de-Abreu; Stevens Rehen

doi:10.1016/j.scr.2016.05.017

Stem Cell Research (Jul 2016)

Generation of iPS cell lines from schizophrenia patients using a non-integrative method

Jaroslaw Sochacki,
Sylvie Devalle,
Marcelo Reis,
Renata de Moraes Maciel,
Bruna da Silveira Paulsen,
Helena Brentani,
Paulo Silva Belmonte-de-Abreu,
Stevens Rehen

Affiliations

Jaroslaw Sochacki: D'Or Institute for Research and Education (IDOR), Rua Diniz Cordeiro, 30, Rio de Janeiro 222281, Brazil
Sylvie Devalle: D'Or Institute for Research and Education (IDOR), Rua Diniz Cordeiro, 30, Rio de Janeiro 222281, Brazil
Marcelo Reis: D'Or Institute for Research and Education (IDOR), Rua Diniz Cordeiro, 30, Rio de Janeiro 222281, Brazil
Renata de Moraes Maciel: D'Or Institute for Research and Education (IDOR), Rua Diniz Cordeiro, 30, Rio de Janeiro 222281, Brazil
Bruna da Silveira Paulsen: Institute of Biomedical Sciences, Federal University of Rio de Janeiro (UFRJ), Avenida Carlos Chagas, 373, Rio de Janeiro 21941, Brazil
Helena Brentani: Department of Psychiatry, Faculty of Medicine, São Paulo University (USP), Avenida Doutor Arnaldo, 455 – Cerqueira César, 01246-903 São Paulo, Brazil
Paulo Silva Belmonte-de-Abreu: Department of Psychiatry, Faculty of Medicine, Federal University of Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos 2400 – Floresta, Porto Alegre 90035-002, Brazil
Stevens Rehen: D'Or Institute for Research and Education (IDOR), Rua Diniz Cordeiro, 30, Rio de Janeiro 222281, Brazil

DOI: https://doi.org/10.1016/j.scr.2016.05.017
Journal volume & issue: Vol. 17, no. 1
pp. 97 – 101

Abstract

Read online

Skin biopsies were collected from three male patients (age 45, 44 and 44) with clinically diagnosed schizophrenia. The patients were diagnosed according to DSM-5 criteria by a trained psychiatrist. Dermal fibroblast cell lines were established and expanded for subsequent reprogramming procedures. Induced pluripotent stem (iPS) cells were derived using the integration-free CytoTune®-iPS 2.0 Sendai Reprogramming Kit, containing Sendai virus particles of the four Yamanaka factors Oct3/4, Sox2, Klf4 and c-Myc.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal