Rapid detection of <it>sacbrood virus </it>(SBV) by one-step reverse transcription loop-mediated isothermal amplification assay

Abstract

Abstract Background Sacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required. Findings A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV. The data demonstrated that, in a simple water bath, SBV RNA could be detected as early as 20 min at 65°C, and a positive amplification reaction was visible to the naked eye due to a color change brought on by the addition of nucleic acid stain SYBR Green. Conclusions The current study presents a method for the rapid and simple detection of SBV by RT-LAMP with high sensitivity and analytic specificity.

Keywords

• <it>Sacbrood virus</it>
• Loop-mediated isothermal amplification
• SYBR Green
• Honeybee