Frontiers in Cellular Neuroscience (Mar 2024)
Inhibition of phospholipase D promotes neurological function recovery and reduces neuroinflammation after spinal cord injury in mice
- Han Ke,
- Han Ke,
- Han Ke,
- Han Ke,
- Han Ke,
- Han Ke,
- Fan Bai,
- Fan Bai,
- Fan Bai,
- Fan Bai,
- Fan Bai,
- Zihan Li,
- Zihan Li,
- Zihan Li,
- Zihan Li,
- Zihan Li,
- Yanbing Zhu,
- Chunjia Zhang,
- Chunjia Zhang,
- Chunjia Zhang,
- Chunjia Zhang,
- Chunjia Zhang,
- Yan Li,
- Yan Li,
- Yan Li,
- Yan Li,
- Yan Li,
- Zuliyaer Talifu,
- Zuliyaer Talifu,
- Zuliyaer Talifu,
- Zuliyaer Talifu,
- Zuliyaer Talifu,
- Zuliyaer Talifu,
- Yunzhu Pan,
- Yunzhu Pan,
- Yunzhu Pan,
- Yunzhu Pan,
- Yunzhu Pan,
- Yunzhu Pan,
- Wubo Liu,
- Wubo Liu,
- Wubo Liu,
- Wubo Liu,
- Wubo Liu,
- Wubo Liu,
- Xin Xu,
- Xin Xu,
- Xin Xu,
- Xin Xu,
- Xin Xu,
- Feng Gao,
- Feng Gao,
- Feng Gao,
- Feng Gao,
- Feng Gao,
- Degang Yang,
- Degang Yang,
- Degang Yang,
- Degang Yang,
- Degang Yang,
- Liangjie Du,
- Liangjie Du,
- Liangjie Du,
- Liangjie Du,
- Liangjie Du,
- Yan Yu,
- Yan Yu,
- Yan Yu,
- Yan Yu,
- Yan Yu,
- Jianjun Li,
- Jianjun Li,
- Jianjun Li,
- Jianjun Li,
- Jianjun Li,
- Jianjun Li,
- Jianjun Li
Affiliations
- Han Ke
- Shandong University, Jinan, Shandong, China
- Han Ke
- China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing, China
- Han Ke
- University of Health and Rehabilitation Sciences, Qingdao, Shandong, China
- Han Ke
- China Rehabilitation Science Institute, Beijing, China
- Han Ke
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Han Ke
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Fan Bai
- China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing, China
- Fan Bai
- China Rehabilitation Science Institute, Beijing, China
- Fan Bai
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Fan Bai
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Fan Bai
- School of Rehabilitation, Capital Medical University, Beijing, China
- Zihan Li
- China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing, China
- Zihan Li
- China Rehabilitation Science Institute, Beijing, China
- Zihan Li
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Zihan Li
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Zihan Li
- School of Rehabilitation, Capital Medical University, Beijing, China
- Yanbing Zhu
- Beijing Clinical Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, China
- Chunjia Zhang
- China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing, China
- Chunjia Zhang
- China Rehabilitation Science Institute, Beijing, China
- Chunjia Zhang
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Chunjia Zhang
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Chunjia Zhang
- School of Rehabilitation, Capital Medical University, Beijing, China
- Yan Li
- China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing, China
- Yan Li
- China Rehabilitation Science Institute, Beijing, China
- Yan Li
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Yan Li
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Yan Li
- School of Rehabilitation, Capital Medical University, Beijing, China
- Zuliyaer Talifu
- China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing, China
- Zuliyaer Talifu
- University of Health and Rehabilitation Sciences, Qingdao, Shandong, China
- Zuliyaer Talifu
- China Rehabilitation Science Institute, Beijing, China
- Zuliyaer Talifu
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Zuliyaer Talifu
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Zuliyaer Talifu
- School of Rehabilitation, Capital Medical University, Beijing, China
- Yunzhu Pan
- China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing, China
- Yunzhu Pan
- University of Health and Rehabilitation Sciences, Qingdao, Shandong, China
- Yunzhu Pan
- China Rehabilitation Science Institute, Beijing, China
- Yunzhu Pan
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Yunzhu Pan
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Yunzhu Pan
- School of Rehabilitation, Capital Medical University, Beijing, China
- Wubo Liu
- Shandong University, Jinan, Shandong, China
- Wubo Liu
- China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing, China
- Wubo Liu
- University of Health and Rehabilitation Sciences, Qingdao, Shandong, China
- Wubo Liu
- China Rehabilitation Science Institute, Beijing, China
- Wubo Liu
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Wubo Liu
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Xin Xu
- China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing, China
- Xin Xu
- China Rehabilitation Science Institute, Beijing, China
- Xin Xu
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Xin Xu
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Xin Xu
- School of Rehabilitation, Capital Medical University, Beijing, China
- Feng Gao
- China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing, China
- Feng Gao
- China Rehabilitation Science Institute, Beijing, China
- Feng Gao
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Feng Gao
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Feng Gao
- School of Rehabilitation, Capital Medical University, Beijing, China
- Degang Yang
- China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing, China
- Degang Yang
- China Rehabilitation Science Institute, Beijing, China
- Degang Yang
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Degang Yang
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Degang Yang
- School of Rehabilitation, Capital Medical University, Beijing, China
- Liangjie Du
- China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing, China
- Liangjie Du
- China Rehabilitation Science Institute, Beijing, China
- Liangjie Du
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Liangjie Du
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Liangjie Du
- School of Rehabilitation, Capital Medical University, Beijing, China
- Yan Yu
- China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing, China
- Yan Yu
- China Rehabilitation Science Institute, Beijing, China
- Yan Yu
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Yan Yu
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Yan Yu
- School of Rehabilitation, Capital Medical University, Beijing, China
- Jianjun Li
- China Rehabilitation Research Center, Beijing Bo’ai Hospital, Beijing, China
- Jianjun Li
- Shandong University, Jinan, Shandong, China
- Jianjun Li
- University of Health and Rehabilitation Sciences, Qingdao, Shandong, China
- Jianjun Li
- China Rehabilitation Science Institute, Beijing, China
- Jianjun Li
- Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China
- Jianjun Li
- Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing, China
- Jianjun Li
- School of Rehabilitation, Capital Medical University, Beijing, China
- DOI
- https://doi.org/10.3389/fncel.2024.1352630
- Journal volume & issue
-
Vol. 18
Abstract
IntroductionSpinal cord injury (SCI) is a severely disabling disease. Hyperactivation of neuroinflammation is one of the main pathophysiological features of secondary SCI, with phospholipid metabolism playing an important role in regulating inflammation. Phospholipase D (PLD), a critical lipid-signaling molecule, is known to be involved in various physiological processes, including the regulation of inflammation. Despite this knowledge, the specific role of PLD in SCI remains unclear.MethodsIn this study, we constructed mouse models of SCI and administered PLD inhibitor (FIPI) treatment to investigate the efficacy of PLD. Additionally, transcriptome sequencing and protein microarray analysis of spinal cord tissues were conducted to further elucidate its mechanism of action.ResultsThe results showed that PLD expression increased after SCI, and inhibition of PLD significantly improved the locomotor ability, reduced glial scarring, and decreased the damage of spinal cord tissues in mice with SCI. Transcriptome sequencing analysis showed that inhibition of PLD altered gene expression in inflammation regulation. Subsequently, the protein microarray analysis of spinal cord tissues revealed variations in numerous inflammatory factors. Biosignature analysis pointed to an association with immunity, thus confirming the results obtained from transcriptome sequencing.DiscussionCollectively, these observations furnish compelling evidence supporting the anti-inflammatory effect of FIPI in the context of SCI, while also offering important insights into the PLD function which may be a potential therapeutic target for SCI.
Keywords
- spinal cord injury
- phospholipase D
- neuroinflammation
- transcriptome sequencing analysis
- protein microarray analysis
