Aged Human Multipotent Mesenchymal Stromal Cells Can Be Rejuvenated by Neuron-Derived Neurotrophic Factor and Improve Heart Function After Injury

Hui-Fang Song, MD, PhD; Sheng He, MD, PhD; Shu-Hong Li, MD, MSc; Wen-Juan Yin, MD, MSc; Jun Wu, MD, MSc; Jian Guo, MD, PhD; Zheng-Bo Shao, MD, PhD; Xiao-Yan Zhai, MD, PhD; Hui Gong, MD, PhD; Li Lu, MD, PhD; Fang Wei, MD, PhD; Richard D. Weisel, MD; Jun Xie, MD, PhD; Ren-Ke Li, MD, PhD

doi:10.1016/j.jacbts.2017.07.014

JACC: Basic to Translational Science (Dec 2017)

Aged Human Multipotent Mesenchymal Stromal Cells Can Be Rejuvenated by Neuron-Derived Neurotrophic Factor and Improve Heart Function After Injury

Hui-Fang Song, MD, PhD,
Sheng He, MD, PhD,
Shu-Hong Li, MD, MSc,
Wen-Juan Yin, MD, MSc,
Jun Wu, MD, MSc,
Jian Guo, MD, PhD,
Zheng-Bo Shao, MD, PhD,
Xiao-Yan Zhai, MD, PhD,
Hui Gong, MD, PhD,
Li Lu, MD, PhD,
Fang Wei, MD, PhD,
Richard D. Weisel, MD,
Jun Xie, MD, PhD,
Ren-Ke Li, MD, PhD

Affiliations

Hui-Fang Song, MD, PhD: Department of Anatomy, Shanxi Medical University, Taiyuan, China
Sheng He, MD, PhD: Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Shanxi Medical University, Taiyuan, China
Shu-Hong Li, MD, MSc: Division of Cardiovascular Surgery, Toronto General Research Institute, University Health Network, Toronto, Canada
Wen-Juan Yin, MD, MSc: Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Shanxi Medical University, Taiyuan, China
Jun Wu, MD, MSc: Division of Cardiovascular Surgery, Toronto General Research Institute, University Health Network, Toronto, Canada
Jian Guo, MD, PhD: Division of Cardiovascular Surgery, Toronto General Research Institute, University Health Network, Toronto, Canada
Zheng-Bo Shao, MD, PhD: Division of Cardiovascular Surgery, Toronto General Research Institute, University Health Network, Toronto, Canada
Xiao-Yan Zhai, MD, PhD: Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Shanxi Medical University, Taiyuan, China
Hui Gong, MD, PhD: Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Shanxi Medical University, Taiyuan, China
Li Lu, MD, PhD: Department of Anatomy, Shanxi Medical University, Taiyuan, China
Fang Wei, MD, PhD: First Hospital of Shanxi Medical University, Taiyuan, China
Richard D. Weisel, MD: Division of Cardiovascular Surgery, Toronto General Research Institute, University Health Network, Toronto, Canada
Jun Xie, MD, PhD: Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Shanxi Medical University, Taiyuan, China
Ren-Ke Li, MD, PhD: Division of Cardiovascular Surgery, Toronto General Research Institute, University Health Network, Toronto, Canada

DOI: https://doi.org/10.1016/j.jacbts.2017.07.014
Journal volume & issue: Vol. 2, no. 6
pp. 702 – 716

Abstract

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Reduced regenerative capacity of aged stem cells hampers the benefits of autologous cell therapy for cardiac regeneration. This study investigated whether neuron-derived neurotrophic factor (NDNF) could rejuvenate aged human bone marrow (hBM)- multipotent mesenchymal stromal cells (MSCs) and whether the rejuvenated hBM-MSCs could improve cardiac repair after ischemic injury. Over-expression of NDNF in old hBM-MSCs decreased cell senescence and apoptosis. Engraftment of NDNF over-expressing old hBM-MSCs into the ischemic area of mouse hearts resulted in improved cardiac function after myocardial infarction, while promoting implanted stem cell survival. Our findings suggest NDNF could be a new factor to rejuvenate aged stem cells and improve their capability to repair the aged heart after injury.

Published in JACC: Basic to Translational Science

ISSN: 2452-302X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Diseases of the circulatory (Cardiovascular) system
Website: https://www.journals.elsevier.com/jacc-basic-to-translational-science/

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