Effect of pectinase on the production of sesquiterpene lactones in the hairy root culture of Lactuca virosa L.

Abstract

Direct addition of pectinase as an elicitor to the hairy root culture of Lactuca virosa L. transformed with Agrobacterium rhizogenes stimulates the accumulation of constitutive sesquiterpene lactones - lactuside A and crepidiaside B. The highest amounts of lactuside A (80% above the control level) and crepidiaside B (39% above the control level) have been found 24 and 48 hours, respectively, after pectinase treatment.

Keywords

• Lactuca virosa L.
• Asteraceae
• hairy root culture
• Agrobacterium rhizogenes
• pectinase
• elicitor
• lactuside A
• crepidiaside B
• sesquiterpene lactones