MMP20 Expression in Chorionic Tissue and Decidua of Women with Early Pregnancy Loss

Abstract

MMPs play an important role in human reproduction, because in the early stages of embryogenesis the degradation and remodeling of extracellular matrix are the key processes. Only known function for MMP20 is dental enamel formation. But the data about its role in spontaneous abortion is very poor. Thus, the aim of this study was to investigate the association of Val275Ala (rs1784423) and A320C (rs2245803) polymorphisms MMP20 gene with pregnancy loss in the first trimester in women, and MMP20 gene expression analysis in decidua and chorionic tissues. A total of 132 women with spontaneous abortion in the first trimester and 144 control patients were examined by a case-control methodology. Samples of chorionic tissue and decidua were taken after surgical termination of normally progressing pregnancies in 5-9 week of gestation (n=12) and spontaneous abortion in 5-9 week of gestation (n=12). Our investigation have not detected association of gene polymorphisms MMP20 Val275Ala (rs1784423) and A320C (rs2245803) with miscarriage in the first trimester. Compared with chorionic tissue, the MMP20 expression was increased in decidua in both groups (p = 0.0037, P = 0.014 respectively). The results demonstrated the tissue-specific MMP20 expression in normal pregnancy and spontaneous abortion.

Keywords

• MMP20
• Gene expression
• Spontaneous abortion
• Chorionic tissue
• Decidua