In-Vivo study of nano chitosan as therapeutic agent for toxic metal implant

Abstract

Chromium (Cr6+) toxicity is a well-known problem in environmental health and safety Cr6+ can cause respiratory disorders, skin irritation, cancer, and kidney disorders. During implantation, cases of biocorrosion and metallosis have occurred due to Cr6+ contact and exposure. Biosorption materials such as chitosan are a promising therapeutic agent to adsorb Cr6+ toxins in the body, as well as aiding the healing process for damaged internal organs. In-vivo studies between relationship of Cr6+ toxicity and chitosan concentration are investigated to recovery progress. We exhibited that Cr6+ can caused severe toxicity and induce death in rats due to organ damage and failure. Meanwhile, nano chitosan was able to adsorb Cr6+ via detection of blood. Recovery of internal organ damage was boosted through therapeutic low molecular size (385 Da) nano chitosan, which we were able to confirm from X-ray images within six weeks of treatment. Average particle size and particles distribution of nano chitosan were influenced by the concentration of nano chitosan powder and dilution solvent. However, we were unable to obtain linear correlations due to the in-vivo condition.

Keywords

• Absorption
• In-vivo
• Ion Cr6+
• Low molecular weight
• Nano chitosan
• Toxicity