7-(1-Methyl-3-Pyrrolyl-)-4,6-Dinitrobenzofuroxan Reduces the Frequency of Antibiotic Resistance Mutations Induced by Ciprofloxacin in Bacteria

Abstract

The aim of the present study was to investigate biological properties of the novel nitrobenzoxadiazole derivative 7-(1-methyl-3-pyrrolyl-)-4,6-dinitrobenzofuroxan. Materials and Methods: We used a bioluminescent test based on a set of lux-biosensors, which are genetically modified E.coli strains able to react on different types of factors that can induce an SOS-response with light emission. The spontaneous and induced mutation frequencies of antibiotic resistance in E. coli were determined by methods of classical genetics of microorganisms. Results: 7-(1-methyl-3-pyrrolyl-)-4,6-dinitrobenzofuroxan demonstrated inhibition of SOS-response in a biosensor model system and significantly reduced the frequency of spontaneous mutations and mutations induced by ciprofloxacin of antibiotic resistance. Conclusion: Based on our data, we can recommend using compound-1 as a starting point for the development of drugs that block mutagenesis associated with the emergence of antibiotic-resistant bacteria.

Keywords

• antibioitic resistance
• fluoroquinolones
• SOS-response
• SOS-inhibitors
• nitrobenzoxadiazole derivatives
• antimutagens