Evaluation of DNA extraction methods and detection of genetically modified organisms in processed foods derived from corn

Abstract

Given the advances in biotechnology cultivation of genetically modified crops, for example, maize (Zea mays) plants increased considerably in recent years. Although such technology presents proven benefits in relation to increased productivity and durability of food, the population still fears to consume genetically modified products. The objective of this study was to compare two protocols based on the use of CTAB and evaluate which is best for extraction of DNA in processed food derived from maize. As well as identifying two of the most common transgenic residues in foodstuffs derived from maize: Cry1Ab and Cry1F. Para this, 14 samples derived from maize were evaluated using two different protocols for DNA extraction and detection of transgenic events conducted by qualitative PCR. Among the samples, 57% resulted positive for detection of both transgenic corn event evaluated.

Keywords

• resíduos de transgênicos, legislação, milho geneticamente modificado, PCR