Effect of non-autologous adipose-derived stem cells transplantation and nerve growth factor on the repair of crushed sciatic nerve in rats

Journal of Birjand University of Medical Sciences. 2014;20(4):357-365

 

Journal Homepage

Journal Title: Journal of Birjand University of Medical Sciences

ISSN: 1607-2197 (Print); 2423-6152 (Online)

Publisher: Birjand University of Medical Sciences and Health Services

Society/Institution: Birjand University of Medical Sciences and Health Services

LCC Subject Category: Medicine: Medicine (General)

Country of publisher: Iran, Islamic Republic of

Language of fulltext: Persian

Full-text formats available: PDF, XML

 

AUTHORS

Azadeh Tajik ( Islamic Azad University, North Tehran Branch, Tehran, Iran. )
Mahnaz Azarnia ( Department of Biology, Faculty of Basic Sciences, Kharazmi University, Tehran, Iran. )
Gholamreza Kaka ( Neuroscience Research Center of Baqiyatallah university of Medical Sciences, Tehran, Iran )
Seyed Homayoon Sadraie ( Neuroscience Research Center of Baqiyatallah university of Medical Sciences, Tehran, Iran. )
Amid Khatiby Aghda ( Department of Physical Medicine and Rehabilitation, Baqiyatallah university of Medical Sciences, Tehran, Iran. )

EDITORIAL INFORMATION

Double blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 18 weeks

 

Abstract | Full Text

Background and Aim: Application of stem cells derived from adipose tissue is very easy. The present study evaluated the effect of transplantation of non-autolugus of adipose derived stem cell (ADSc) and nerve growth factor (NGF) in repairing of crushed sciatic nerve in rats by examining their behavior and electrophysiology. Materials and Methods: Twenty-eight adult male rats were used in this study. The animals were randomly divided into four equal groups: (healthy rats), as control(sciatic nerve crushed rats), (sciatic nerve crushed rats treated with NGF), and (sciatic nerve crushed rats treated by means of transplantation of non-autolugue of ADSc).Then,the improvement of sensory function and sciatic nerve index (SFI) of the groups were assessed. Results: Time of recovery of sensory parameters after two weeks treatment with NGF significantly decreased when compared to the control group (P<0.01), while the cell therapy groupshowed siqnificant improvement. At the end of the sixth week, mean of SFI in cell therapy and NGF groups increased compared to the control group but the differences were not significant. Mean NCV and AMP also showed a non-significant increase in the cell therapy group compared to the control group. Conclusion: Transplantation of non-autologous of adipose-derived stem cells (ASDc) is an appropriate therapeutic approach in repairing of neurological injuries and NGF has a positive effect in crushed sciatic nerve regeneration.