The effects of casein kinase 2 interacting protein-1 on the growth and development of craniomaxillofacial soft and hard tissues in mice
TANG Mingyue,
HU Ao,
CAI Bolei,
GAO Ye,
LIU Fuwei,
LV Qianxin,
JIN Dan,
HOU Yan,
WANG Le,
ZHANG Zhouyang,
KONG Liang
Affiliations
TANG Mingyue
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Department Oral and Maxillofacial Surgery, Stomatological hospital, Air Force Medical University
HU Ao
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Department Oral and Maxillofacial Surgery, Stomatological hospital, Air Force Medical University
CAI Bolei
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Department Oral and Maxillofacial Surgery, Stomatological hospital, Air Force Medical University
GAO Ye
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Department Oral and Maxillofacial Surgery, Stomatological hospital, Air Force Medical University
LIU Fuwei
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Department Oral and Maxillofacial Surgery, Stomatological hospital, Air Force Medical University
LV Qianxin
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Department Oral and Maxillofacial Surgery, Stomatological hospital, Air Force Medical University
JIN Dan
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Department Oral and Maxillofacial Surgery, Stomatological hospital, Air Force Medical University
HOU Yan
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Department Oral and Maxillofacial Surgery, Stomatological hospital, Air Force Medical University
WANG Le
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Department Oral and Maxillofacial Surgery, Stomatological hospital, Air Force Medical University
ZHANG Zhouyang
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Department Oral and Maxillofacial Surgery, Stomatological hospital, Air Force Medical University
KONG Liang
State Key Laboratory of Military Stomatology, National Clinical Research Center for Oral Diseases, Shanxi Clinical Research Center for Oral Diseases, Department Oral and Maxillofacial Surgery, Stomatological hospital, Air Force Medical University
Objective To investigate the effect of casein kinase 2 interacting protein-1 (CKIP-1) on craniofacial soft tissues and hard tissues, to provide the basis for the study and treatment of craniomaxillofacial related diseases. Methods 6-month- old male CKIP-1 knockout (KO) mice were selected as the experimental group, and wild-type (WT) mice were selected as the control group. The craniomaxillofacial hard tissues (parietal bone, nasal bone, incisors and molars) were analyzed through micro- CT, and the morphological changes of maxillofacial soft tissues (nasal cartilage, lip mucosa and tongue) were analyzed through HE staining and toluidine blue staining. Results CKIP-1 negatively regulated bone mass of cancellous bone of cranial and maxillofacial bones and dentin mineralization. Compared with the WT mice, the thickness of the parietal baffle layer increased by 93% in KO mice, while cortical bone showed no significant difference between the two groups. The nasal cancellous bone thickness increased by 160% in KO-mice, while cortical bone showed no significant difference between the two groups; the enamel thickness was normal, but the pulp cavity became smaller and the dentin thickness increased by 48%. Compared with the WT mice, the HE staining and toluidine blue staining analyses of the soft tissues revealed that the thickness of the alar cartilage plate of KO mice increased by 57%, and local ossification was found within the cartilage plate. The thickness of the keratinized layer of the labial mucosa increased by 170% in KO mice and the muscle fiber diameter of the lingual muscle increased by 45%. Conclusion CKIP-1 genes have different effects on the growth and development of various soft and hard tissues in the maxillofacial region of mice.
