Generation of induced pluripotent stem cell line (IPTi002-A) from an 87-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype

Lin Zhang; Qiao Ren; Xiaohui Liu; Gang Liu; Shulin Meng; Kang Xiahou; Yongxiang Zhang; Ning Jiang; Wenxia Zhou

Stem Cell Research (May 2021)

Generation of induced pluripotent stem cell line (IPTi002-A) from an 87-year old sporadic Alzheimer's disease patient with APOE3 (ε3/ε3) genotype

Lin Zhang,
Qiao Ren,
Xiaohui Liu,
Gang Liu,
Shulin Meng,
Kang Xiahou,
Yongxiang Zhang,
Ning Jiang,
Wenxia Zhou

Affiliations

Lin Zhang: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, China; Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China
Qiao Ren: Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China
Xiaohui Liu: Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China
Gang Liu: Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China
Shulin Meng: IxCell BIOTECHNOLOGY Co.,Ltd, Shanghai, China
Kang Xiahou: IxCell BIOTECHNOLOGY Co.,Ltd, Shanghai, China
Yongxiang Zhang: School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang, China; Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China; Corresponding authors at: Shenyang Pharmaceutical University; 103 Wenhua Road, 110016, Shenyang, PR China (Y. Zhang). Beijing Institute of Pharmacology and Toxicology; 27 Taiping Road, 100850, Beijing, PR China (N. Jiang and W. Zhou).
Ning Jiang: Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China; Corresponding authors at: Shenyang Pharmaceutical University; 103 Wenhua Road, 110016, Shenyang, PR China (Y. Zhang). Beijing Institute of Pharmacology and Toxicology; 27 Taiping Road, 100850, Beijing, PR China (N. Jiang and W. Zhou).
Wenxia Zhou: Beijing Institute of Pharmacology and Toxicology, Beijing, China; State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China; Corresponding authors at: Shenyang Pharmaceutical University; 103 Wenhua Road, 110016, Shenyang, PR China (Y. Zhang). Beijing Institute of Pharmacology and Toxicology; 27 Taiping Road, 100850, Beijing, PR China (N. Jiang and W. Zhou).

Journal volume & issue: Vol. 53
p. 102282

Abstract

Read online

Here, we described the generation of human induced pluripotent stem cells (iPSC) from peripheral blood mononuclear cells (PBMCs) of a 87-year-old female patient with sporadic Alzheimer's disease (sAD) having APOE3 (ε3/ε3) genotype. iPSC line were generated from PBMCs with four factors of OCT4, SOX2, c-MYC and KLF4 using episomal system. The pluripotency of the iPSC line was assessed by embryoid body (EB) formation. Flow cytometry analyses revealed >97% cells positive for the pluripotency markers NANOG, OCT4 and SSEA4. Furthermore, the iPSC line displayed a normal karyotype (46, XX). The iPSC line may provide valuable tools for the study of sAD pathogenesis.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal