Effects of Long Non-coding RNA-MALAT1 on Proliferation and Apoptosis of Prostate Cancer Cells

Abstract

Objective To investigate the effect of long non-coding RNA-MALAT1 on the proliferation and apoptosis of hormone-dependent prostate cancer cells and possible mechanisms. Methods MALAT1 overexpression and interference treatment were performed in the hormone-dependent prostate cancer PC3 and LNCaP cell lines. MTT assay, flow cytometry, Matrigel and clone formation were used to examine the effects of MALAT1 overexpression and interference on cell biological behavior. Western blot was used to evaluate the effects of MALAT1 on the expression of Cyclin D1, Bcl-2, Bax and p-ERK1/2. Results Compared with control group, MALAT1 group cells showed enhanced abilities of proliferation (P < 0.01), clone formation and angiogenesis; the cells number in the G0/G1 phase was increased; the apoptotic rate was decreased. In contrast, the cells in si-MALAT1 group showed significantly lower proliferation ability compared with control group (P < 0.01); the abilities of clone formation and angiogenesis were reduced; the number of cells in the G0/G1 phase was decreased, while apoptotic rate was increased. Conclusion MALAT1 promotes the occurrence and development of prostate cancer. The inhibition of MALAT1 expression may play important roles in the treatment of prostate cancer.

Keywords

• long non-coding rna
• malat1
• prostate cancer
• cell proliferation
• cell apoptosis