Determinação de proteínas totais via espectrofometria: vantagens e desvantagens dos métodos existentes Determination of total protein by spectrophotometry: advantages and disadvantages of proposed methods

Abstract

Spectrophotometric determination of total protein is used in several areas such as clinical analysis, food science and technology, biochemistry, protein chemistry, physiology. Five spectrophotometric methods are mostly used: biuret, Lowry, Bradford, Smith and UV absorption. In this review a general overview of these methods is presented (interferences, applications); other methodologies are also discussed.

Keywords

• protein
• spectrophotometry
• protein analysis