Asian Journal of Urology (Jan 2019)
Application of fluorescence in situ hybridization in the detection of bladder transitional-cell carcinoma: A multi-center clinical study based on Chinese population
- Liqun Zhou,
- Kaiwei Yang,
- Xuesong Li,
- Yi Ding,
- Dawei Mu,
- Hanzhong Li,
- Yong Yan,
- Jinyi Li,
- Dongwen Wang,
- Wei Li,
- Yulong Cong,
- Jiangping Gao,
- Kewei Ma,
- Yajun Xiao,
- Sheng Zhang,
- Hongyi Jiang,
- Weilie Hu,
- Qiang Wei,
- Xunbo Jin,
- Zhichen Guan,
- Qingyong Liu,
- Danfeng Xu,
- Xin Gao,
- Yongguang Jiang,
- Weimin Gan,
- Guang Sun,
- Qing Wang,
- Yanhui Liu,
- Jianquan Hou,
- Liping Xie,
- Xishuang Song,
- Fengshuo Jin,
- Jiafu Feng,
- Ming Cai,
- Zhaozhao Liang,
- Jie Zhang,
- Dingwei Ye,
- Lin Qi,
- Lulin Ma,
- Jianzhong Shou,
- Yuping Dai,
- Jianyong Shao,
- Ye Tian,
- Shizhe Hong,
- Tao Xu,
- Chuize Kong,
- Zefeng Kang,
- Yuexin Liu,
- Xun Qu,
- Benkang Shi,
- Shaobin Zheng,
- Yi Lin,
- Shujie Xia,
- Dong Wei,
- Jianbo Wu,
- Weiling Fu,
- Zhiping Wang,
- Jianbo Liang
Affiliations
- Liqun Zhou
- Department of Urology, Peking University First Hospital, Beijing, China; Institute of Urology, Peking University, Beijing, China; National Urological Cancer Center, Beijing, China; Corresponding author. Department of Urology, Peking University First Hospital, Institute of Urology, Peking University, National Urological Cancer Center, Beijing, China.
- Kaiwei Yang
- Department of Urology, Peking University First Hospital, Beijing, China; Institute of Urology, Peking University, Beijing, China; National Urological Cancer Center, Beijing, China
- Xuesong Li
- Department of Urology, Peking University First Hospital, Beijing, China; Institute of Urology, Peking University, Beijing, China; National Urological Cancer Center, Beijing, China
- Yi Ding
- Department of Urology, Peking University First Hospital, Beijing, China; Institute of Urology, Peking University, Beijing, China; National Urological Cancer Center, Beijing, China
- Dawei Mu
- Air Force General Hospital, PLA, Beijing, China
- Hanzhong Li
- Department of Urology, Peking Union Medical College Hospital, Beijing, China
- Yong Yan
- Department of Urology, Beijing Chao-Yang Hospital, Beijing, China
- Jinyi Li
- Department of Urology, Armed-Police General Hospital, Beijing, China
- Dongwen Wang
- Department of Urology, Shanxi Medical University First Hospital, Taiyuan, China
- Wei Li
- Department of Urology, Hebei Medical University Second Hospital, Shijiazhuang, China
- Yulong Cong
- Department of Urology, The General Hospital of the People's Liberation Army, Beijing, China
- Jiangping Gao
- Department of Urology, The General Hospital of the People's Liberation Army, Beijing, China
- Kewei Ma
- Department of Urology, First Affiliated Hospital of Jilin University, Changchun, China
- Yajun Xiao
- Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
- Sheng Zhang
- Department of Pathology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China
- Hongyi Jiang
- Department of Urology, The Second Xiangya Hospital of Central South University, Changsha, China
- Weilie Hu
- Department of Urology, General Hospital of Guangzhou Military Command of PLA, Guangzhou, China
- Qiang Wei
- Department of Urology, West China School of Medicine, Chengdu, China
- Xunbo Jin
- Department of Urology, Shangdong Province-owned Hospital, Jinan, China
- Zhichen Guan
- Department of Urology, Peking University Shenzhen Hospital, Shenzhen, China
- Qingyong Liu
- Department of Urology, The Central Hospital of Jinan City, Jinan, China
- Danfeng Xu
- Department of Urology, Shanghai Changzheng Hospital, Shanghai, China
- Xin Gao
- Department of Urology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
- Yongguang Jiang
- Department of Urology, Beijing Anzhen Hospital of Capital Medical University, Beijing, China
- Weimin Gan
- Department of Urology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China
- Guang Sun
- Department of Urology, The Second Hospital of Tianjin Medical University, Tianjin, China
- Qing Wang
- Department of Urology, People's Hospital of Xinjiang Uygur Autonomous Region, Wulumuqi, China
- Yanhui Liu
- Department of Urology, Guangdong General Hospital, Guangzhou, China
- Jianquan Hou
- Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, China
- Liping Xie
- Department of Urology, The First Hospital of Zhejiang Province, Hangzhou, China
- Xishuang Song
- Department of Urology, The First Affiliated Hospital of Dalian Medical University, Dalian, China
- Fengshuo Jin
- Department of Urology, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing, China
- Jiafu Feng
- Department of Urology, Mianyang Central Hospital, Mianyang, China
- Ming Cai
- Department of Urology, General Hospital of PLA General Staff Headquarters, Beijing, China
- Zhaozhao Liang
- Department of Urology, First Affiliated Hospital of Anhui Medical University, Hefei, China
- Jie Zhang
- Department of Urology, Renmin Hospital of Wuhan University, Wuhan, China
- Dingwei Ye
- Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, China
- Lin Qi
- Department of Urology, Xiangya Hospital of Central South University, Changsha, China
- Lulin Ma
- Department of Urology, Peking University Third Hospital, Beijing, China
- Jianzhong Shou
- Department of Urology, Cancer Hospital of Chinese Academy of Medical Sciences, Beijing, China
- Yuping Dai
- Department of Urology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
- Jianyong Shao
- Department of Urology, Sun Yat-sen University Cancer Center, Guangzhou, China
- Ye Tian
- Department of Urology, Beijing Friendship Hospital of Capital Medical University, Beijing, China
- Shizhe Hong
- Department of Urology, Wenzhou Central Hospital, Wenzhou, China
- Tao Xu
- Department of Urology, Peking University People's Hospital, Beijing, China
- Chuize Kong
- Department of Urology, The First Hospital of China Medical University, Shenyang, China
- Zefeng Kang
- Department of Urology, Qinghai Province People's Hospital, Xining, China
- Yuexin Liu
- Department of Urology, Beijing Tongren Hospital of Capital Medical University, Beijing, China
- Xun Qu
- Department of Urology, Qilu Hospital of Shandong University, Jinan, China
- Benkang Shi
- Department of Urology, Qilu Hospital of Shandong University, Jinan, China
- Shaobin Zheng
- Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, China
- Yi Lin
- Department of Urology, Tianjing Medical University General Hospital, Tianjin, China
- Shujie Xia
- Department of Urology, Shanghai General Hospital, Shanghai, China
- Dong Wei
- Department of Urology, Beijing Hospital, Beijing, China
- Jianbo Wu
- Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
- Weiling Fu
- Department of Urology, Southwest Hospital, Chongqing, China
- Zhiping Wang
- Department of Urology, Lanzhou University Second Hospital, Lanzhou, China
- Jianbo Liang
- Department of Urology, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, China
- Journal volume & issue
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Vol. 6,
no. 1
pp. 114 – 121
Abstract
Objective: To evaluate the diagnostic value of fluorescence in situ hybridization (FISH) in bladder cancer. Methods: We enrolled healthy volunteers and patients who were clinically suspected to have bladder cancer and conducted FISH tests and cytology examinations from August 2007 to December 2008. Receiver operating characteristic (ROC) curve analysis was performed and the area under curve (AUC) values were calculated for both the FISH and urine cytology tests. Results: A cohort of 988 healthy volunteers was enrolled to establish a reference range for the normal population. A total of 4807 patients with hematuria were prospectively, randomly enrolled for the simultaneous analysis of urine cytology, FISH testing, and a final diagnosis as determined by the pathologic findings of a biopsy or a surgically-excised specimen. Overall, the sensitivity of FISH in detecting transitional-cell carcinoma was 82.7%, while that of cytology was 33.4% (p < 0.001). The sensitivity values of FISH for non-muscle invasive and muscle invasive bladder transitional-cell carcinoma were 81.7% and 89.6%, respectively (p = 0.004). The sensitivity values of FISH for low and high grade bladder cancer were 82.6% and 90.1%, respectively (p = 0.002). Conclusion: FISH is significantly more sensitive than voided urine cytology for detecting bladder cancer in patients evaluated for gross hematuria at all cancer grades and stages. Higher sensitivity using FISH was obtained in high grade and muscle invasive tumors. Keywords: Bladder transitional-cell carcinoma, Fluorescence in situ hybridization, Detection, Grade, Stage
