The effect of the operation conditions and the extraction techniques on the yield, kinetics and composition of methanol extracts of Hieracium pilosella L.

Abstract

The optimal operational extraction conditions were determined by investigating the influence of the methanol concentration, solvomodule and temperature of the maceration extraction on the yield and kinetics of total extractive matter, chlorogenic acid, umbelliferone and apigenin-7-O-glucoside from Hieracium pilosella L. Based on the results of Soxhlet and Tillepape extraction kinetics investigations of the total extractive matter and the components under the optimal maceration operation conditions it was found that the highest yields of the extractive matter and investigated bioactive components extracted from the dry plant material were obtained by using the Soxhlet extraction method. The contents of chlorogenic acid, umbelliferone and apigenin-7-O-glucoside in the extracts were determined by HPLC method. Chlorogenic acid is the component with the highest share in all the extracts.

Keywords

• Hieracium pilosella L.
• Extraction techniques
• HPLC analysis
• Chlorogenic acid
• Umbelliferone
• Apigenin-7-O-glucoside