Construction and evaluation of positive plasmid molecules for adulteration detection of fur animal-derived components in meat products

Abstract

ObjectiveTo establish a rapid detection method for adulteration of fur animal-derived components in meat products.MethodsThree positive plasmids （fox， raccon dog and mink） for detection were constructed， and their specificity， sensitivity and applicability were analyzed by real-time quantitative PCR.ResultsThe constructed positive plasmid molecules of fox， raccoon dog and mink had high specificity and sensitivity， and the sensitivity could reach 10-4 ng/μL. The amplification efficiency of standard curves were 94.451%， 117.461% and 114.709%， respectively. And the correlation coefficient R2 was over 0.995. Three components could be detected in mixed meat samples as well as common meat products， the limits of detection were less than 1%， which had good feasibility and applicability.ConclusionThe three positive plasmids constructed by this method could meet the needs of meat adulteration detection in practical work.

Keywords

• fur animal
• positive plasmid
• specificity
• sensitivity