STAR Protocols (Dec 2023)
Ultrasound-guided lymph node fine-needle aspiration for evaluating post-vaccination germinal center responses in humans
- Larissa L.S. Scholte,
- David J. Leggat,
- Kristen W. Cohen,
- Lara Hoeweler,
- Guacyara C. Erwin,
- Farhard Rahaman,
- Angela Lombardo,
- Vincent Philiponis,
- Dagna S. Laufer,
- Heather Siefers,
- Alexis M. Ruppel,
- Joshua Brand,
- Janine Maenza,
- Rhi Bronson,
- Madhu Prabhakaran,
- Jalen Jean-Baptiste,
- Orpheus Kolokythas,
- Aimee A. Desrosiers,
- Caroline K. Thoreson,
- Antje Heit,
- Nadia J. Khati,
- Elissa Malkin,
- M. Juliana McElrath,
- Adrian B. McDermott,
- William R. Schief,
- David Diemert,
- Jeffrey M. Bethony
Affiliations
- Larissa L.S. Scholte
- Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA; Corresponding author
- David J. Leggat
- The US Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA; Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
- Kristen W. Cohen
- Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA
- Lara Hoeweler
- Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA
- Guacyara C. Erwin
- Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA
- Farhard Rahaman
- IAVI, New York, NY 10004, USA
- Angela Lombardo
- IAVI, New York, NY 10004, USA
- Vincent Philiponis
- IAVI, New York, NY 10004, USA
- Dagna S. Laufer
- IAVI, New York, NY 10004, USA
- Heather Siefers
- IAVI, New York, NY 10004, USA
- Alexis M. Ruppel
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
- Joshua Brand
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
- Janine Maenza
- Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA; Department of Medicine, Division of Allergy and Infectious Disease, University of Washington, Seattle, WA 98195, USA
- Rhi Bronson
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
- Madhu Prabhakaran
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
- Jalen Jean-Baptiste
- Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
- Orpheus Kolokythas
- Department of Radiology, University of Washington, Seattle, WA 98195, USA
- Aimee A. Desrosiers
- Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA
- Caroline K. Thoreson
- Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA
- Antje Heit
- Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA
- Nadia J. Khati
- Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA
- Elissa Malkin
- Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA
- M. Juliana McElrath
- Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA; Department of Medicine, Division of Allergy and Infectious Disease, University of Washington, Seattle, WA 98195, USA
- Adrian B. McDermott
- Vaccines Research and Development, Sanofi, Swiftwater, PA 183709, USA
- William R. Schief
- IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA; Center for HIV/AIDS Vaccine Development, The Scripps Research Institute, La Jolla, CA 92037, USA; Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA; The Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard University, Cambridge, MA 02139, USA
- David Diemert
- Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA
- Jeffrey M. Bethony
- Vaccine Research Unit, The George Washington University, Washington, DC 20037, USA
- Journal volume & issue
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Vol. 4,
no. 4
p. 102576
Abstract
Summary: The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells. We describe steps for media and tube preparation and sample collection using an ultrasound-guided LN fine-needle aspiration procedure. This protocol is safe, quick, low-cost, and less invasive than excisional biopsy.For complete details on the use and execution of this protocol, please refer to Leggat et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
