Andrographolide Inhibits Mechanical and Thermal Hyperalgesia in a Rat Model of HIV-Induced Neuropathic Pain

Zhihua Yi; Zhihua Yi; Zhihua Yi; Zhihua Yi; Shuai Ouyang; Congfa Zhou; Lihui Xie; Zhi Fang; Huilong Yuan; Huilong Yuan; Jinpu Yang; Lifang Zou; Lifang Zou; Tianyu Jia; Tianyu Jia; Shanhong Zhao; Shanhong Zhao; Lin Li; Lin Li; Liran Shi; Liran Shi; Yun Gao; Yun Gao; Guilin Li; Guilin Li; Shuangmei Liu; Shuangmei Liu; Hong Xu; Hong Xu; Changshui Xu; Changshui Xu; Chunping Zhang; Chunping Zhang; Shangdong Liang; Shangdong Liang; Shangdong Liang

doi:10.3389/fphar.2018.00593

Frontiers in Pharmacology (Jun 2018)

Andrographolide Inhibits Mechanical and Thermal Hyperalgesia in a Rat Model of HIV-Induced Neuropathic Pain

Zhihua Yi,
Zhihua Yi,
Zhihua Yi,
Zhihua Yi,
Shuai Ouyang,
Congfa Zhou,
Lihui Xie,
Zhi Fang,
Huilong Yuan,
Huilong Yuan,
Jinpu Yang,
Lifang Zou,
Lifang Zou,
Tianyu Jia,
Tianyu Jia,
Shanhong Zhao,
Shanhong Zhao,
Lin Li,
Lin Li,
Liran Shi,
Liran Shi,
Yun Gao,
Yun Gao,
Guilin Li,
Guilin Li,
Shuangmei Liu,
Shuangmei Liu,
Hong Xu,
Hong Xu,
Changshui Xu,
Changshui Xu,
Chunping Zhang,
Chunping Zhang,
Shangdong Liang,
Shangdong Liang,
Shangdong Liang

Affiliations

Zhihua Yi: Department of Physiology, Medical College of Nanchang University, Nanchang, China
Zhihua Yi: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Zhihua Yi: Nursing College, Medical College of Nanchang University, Nanchang, China
Zhihua Yi: School of Life Sciences, Nanchang University, Nanchang, China
Shuai Ouyang: Undergraduate Student of the Clinical Department, Medical College of Nanchang University, Nanchang, China
Congfa Zhou: Department of Anatomy, Medical College of Nanchang University, Nanchang, China
Lihui Xie: Undergraduate Student of the Clinical Department, Medical College of Nanchang University, Nanchang, China
Zhi Fang: Undergraduate Student of the Clinical Department, Medical College of Nanchang University, Nanchang, China
Huilong Yuan: Department of Physiology, Medical College of Nanchang University, Nanchang, China
Huilong Yuan: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Jinpu Yang: Undergraduate Student of the Queen Mary School, Medical College of Nanchang University, Nanchang, China
Lifang Zou: Department of Physiology, Medical College of Nanchang University, Nanchang, China
Lifang Zou: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Tianyu Jia: Department of Physiology, Medical College of Nanchang University, Nanchang, China
Tianyu Jia: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Shanhong Zhao: Department of Physiology, Medical College of Nanchang University, Nanchang, China
Shanhong Zhao: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Lin Li: Department of Physiology, Medical College of Nanchang University, Nanchang, China
Lin Li: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Liran Shi: Department of Physiology, Medical College of Nanchang University, Nanchang, China
Liran Shi: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Yun Gao: Department of Physiology, Medical College of Nanchang University, Nanchang, China
Yun Gao: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Guilin Li: Department of Physiology, Medical College of Nanchang University, Nanchang, China
Guilin Li: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Shuangmei Liu: Department of Physiology, Medical College of Nanchang University, Nanchang, China
Shuangmei Liu: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Hong Xu: Department of Physiology, Medical College of Nanchang University, Nanchang, China
Hong Xu: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Changshui Xu: Department of Physiology, Medical College of Nanchang University, Nanchang, China
Changshui Xu: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Chunping Zhang: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Chunping Zhang: Department of Cell Biology, Medical College of Nanchang University, Nanchang, China
Shangdong Liang: Department of Physiology, Medical College of Nanchang University, Nanchang, China
Shangdong Liang: Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, China
Shangdong Liang: School of Life Sciences, Nanchang University, Nanchang, China

DOI: https://doi.org/10.3389/fphar.2018.00593
Journal volume & issue: Vol. 9

Abstract

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Aim: In this study, we investigated whether andrographolide (Andro) can alleviate neuropathic pain induced by HIV gp120 plus ddC treatment and the mechanism of its action.Methods: The paw withdrawal threshold and the paw withdrawal latency were observed to assess pain behaviors in all groups of the rats, including control group, control combined with Andro treatment group, sham group, gp120 combined with ddC treatment group, gp120 plus ddC combined with A438079 treatment group, and gp120 plus ddC combined with Andro treatment by intrathecally injecting at a dose of 25 μg/20 μl group. The protein expression levels of the P2X7 receptor, tumor necrosis factor-α-receptor (TNFα-R), interleukin-1β (IL-1β), IL-10, phospho-extracellular regulated protein kinases (ERK) (p-ERK) in the L4–L6 dorsal root ganglia (DRG) were measured by western blotting. Real-time quantitative polymerase chain reaction was used to test the mRNA expression level of the P2X7 receptor. Double-labeling immunofluorescence was used to identify the co-localization of the P2X7 receptor with glial fibrillary acidic protein (GFAP) in DRG. Molecular docking was performed to identify whether the Andro interacted perfectly with the rat P2X7 (rP2X7) receptor.Results: Andro attenuated the mechanical and thermal hyperalgesia in gp120+ddC-treated rats and down-regulated the P2X7 receptor mRNA and protein expression in the L4–L6 DRGs of gp120+ddC-treated rats. Additionally, Andro simultaneously decreased the expression of TNFα-R and IL-1β protein, increased the expression of IL-10 protein in L4–L6 DRGs, and inhibited the activation of ERK signaling pathways. Moreover, Andro decreased the co-expression of GFAP and the P2X7 receptor in the SGCs of L4–L6 DRG on 14th day after surgery.Conclusion: Andro decreased the hyperalgesia induced by gp120 plus ddC.

Published in Frontiers in Pharmacology

ISSN: 1663-9812 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://journal.frontiersin.org/journals/pharmacology

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