Nature Communications (May 2021)
Nanostructure-specific X-ray tomography reveals myelin levels, integrity and axon orientations in mouse and human nervous tissue
- Marios Georgiadis,
- Aileen Schroeter,
- Zirui Gao,
- Manuel Guizar-Sicairos,
- Marianne Liebi,
- Christoph Leuze,
- Jennifer A. McNab,
- Aleezah Balolia,
- Jelle Veraart,
- Benjamin Ades-Aron,
- Sunglyoung Kim,
- Timothy Shepherd,
- Choong H. Lee,
- Piotr Walczak,
- Shirish Chodankar,
- Phillip DiGiacomo,
- Gergely David,
- Mark Augath,
- Valerio Zerbi,
- Stefan Sommer,
- Ivan Rajkovic,
- Thomas Weiss,
- Oliver Bunk,
- Lin Yang,
- Jiangyang Zhang,
- Dmitry S. Novikov,
- Michael Zeineh,
- Els Fieremans,
- Markus Rudin
Affiliations
- Marios Georgiadis
- Institute for Biomedical Engineering, ETH Zurich
- Aileen Schroeter
- Institute for Biomedical Engineering, ETH Zurich
- Zirui Gao
- Institute for Biomedical Engineering, ETH Zurich
- Manuel Guizar-Sicairos
- Swiss Light Source, Paul Scherrer Institute
- Marianne Liebi
- Department of Physics, Chalmers University of Technology
- Christoph Leuze
- Department of Radiology, Stanford School of Medicine
- Jennifer A. McNab
- Department of Radiology, Stanford School of Medicine
- Aleezah Balolia
- Department of Integrative Biology, University of Colorado Denver
- Jelle Veraart
- Center for Biomedical Imaging, New York University School of Medicine
- Benjamin Ades-Aron
- Center for Biomedical Imaging, New York University School of Medicine
- Sunglyoung Kim
- Center for Biomedical Imaging, New York University School of Medicine
- Timothy Shepherd
- Center for Biomedical Imaging, New York University School of Medicine
- Choong H. Lee
- Center for Biomedical Imaging, New York University School of Medicine
- Piotr Walczak
- Department of Radiology, Johns Hopkins Medicine
- Shirish Chodankar
- National Synchrotron Light Source II, Brookhaven National Laboratory
- Phillip DiGiacomo
- Department of Radiology, Stanford School of Medicine
- Gergely David
- Balgrist University Hospital, University of Zurich
- Mark Augath
- Institute for Biomedical Engineering, ETH Zurich
- Valerio Zerbi
- Institute for Biomedical Engineering, ETH Zurich
- Stefan Sommer
- Institute for Biomedical Engineering, ETH Zurich
- Ivan Rajkovic
- Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory
- Thomas Weiss
- Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory
- Oliver Bunk
- Swiss Light Source, Paul Scherrer Institute
- Lin Yang
- National Synchrotron Light Source II, Brookhaven National Laboratory
- Jiangyang Zhang
- Center for Biomedical Imaging, New York University School of Medicine
- Dmitry S. Novikov
- Center for Biomedical Imaging, New York University School of Medicine
- Michael Zeineh
- Department of Radiology, Stanford School of Medicine
- Els Fieremans
- Center for Biomedical Imaging, New York University School of Medicine
- Markus Rudin
- Institute for Biomedical Engineering, ETH Zurich
- DOI
- https://doi.org/10.1038/s41467-021-22719-7
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 13
Abstract
Small-angle X-ray scattering (SAXS) combines the high tissue penetration of X-rays with specificity to periodic nanostructures. The authors use SAXS tensor tomography (SAXS-TT) on intact mouse and human brain tissue samples, to quantify myelin levels and determine myelin integrity, myelinated axon orientation, and fibre tracts non-destructively.
