Green synthesis, characterization and biocompatibility evaluation of silver nanoparticles using radish seeds

Abstract

Green synthesis of silver nanoparticles has attained wider interest for biomedical applications owing to higher stability, and biocompatibility. Unlike the physico-chemical method, green synthesis of nanoparticles is an ecofriendly, economical and simple process. The present study aims to synthesize silver nanoparticles and evaluate its biocompatibility using radish (Raphanus sativus L.) seeds as reducing and stabilizing agent. The silver nanoparticles showed UV–vis absorption peak around 452 nm. The nanoparticles were thereafter characterized using X-ray diffractometer (XRD), Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), and Energy Dispersive X-ray (EDX) analysis. The synthesized nanoparticles revealed crystalline nature, spherical shape, with size in the range of 5–20 nm and strong intense peak around 3 KeV corresponding to elemental silver. The biomolecules of seed extract enable the reduction of silver ions is revealed by specific bands in FTIR spectra. Further, blood compatibility of silver nanoparticles was ascertained using hemolysis assay which revealed non-toxic nature of AgNPs. The biocompatibility of silver nanoparticles was assessed with colon carcinoma cell line HCT-15 using cell viability assay. It showed higher cell compatibility under the safety framework. Hence, these preliminary studies suggest biocompatible properties of silver nanoparticles which is major prerequisite for its biomedical application as drug-carrier system for future cancer therapy.

Keywords

• Silver nanoparticles
• Green synthesis
• Characterization
• Blood compatibility
• Cell compatibility