Frontiers in Microbiology (Nov 2022)

Conserved longitudinal alterations of anti-S-protein IgG subclasses in disease progression in initial ancestral Wuhan and vaccine breakthrough Delta infections

  • Yun Shan Goh,
  • Siew-Wai Fong,
  • Pei Xiang Hor,
  • Siti Naqiah Amrun,
  • Cheryl Yi-Pin Lee,
  • Barnaby Edward Young,
  • Barnaby Edward Young,
  • Barnaby Edward Young,
  • Po Ying Chia,
  • Po Ying Chia,
  • Po Ying Chia,
  • Paul A. Tambyah,
  • Paul A. Tambyah,
  • Shirin Kalimuddin,
  • Shirin Kalimuddin,
  • Surinder Pada,
  • Seow-Yen Tan,
  • Louisa Jin Sun,
  • Mark I-Cheng Chen,
  • Mark I-Cheng Chen,
  • Yee-Sin Leo,
  • Yee-Sin Leo,
  • Yee-Sin Leo,
  • Yee-Sin Leo,
  • Yee-Sin Leo,
  • Yee-Sin Leo,
  • David C. Lye,
  • David C. Lye,
  • David C. Lye,
  • David C. Lye,
  • Lisa F. P. Ng,
  • Lisa F. P. Ng,
  • Lisa F. P. Ng,
  • Laurent Renia,
  • Laurent Renia,
  • Laurent Renia

DOI
https://doi.org/10.3389/fmicb.2022.1043049
Journal volume & issue
Vol. 13

Abstract

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IntroductionCOVID-19 has a wide disease spectrum ranging from asymptomatic to severe. While humoral immune responses are critical in preventing infection, the immune mechanisms leading to severe disease, and the identification of biomarkers of disease progression and/or resolution of the infection remains to be determined.MethodsPlasma samples were obtained from infections during the initial wave of ancestral wildtype SARS-CoV-2 and from vaccine breakthrough infections during the wave of Delta variant, up to six months post infection. The spike-specific antibody profiles were compared across different severity groups and timepoints.ResultsWe found an association between spike-specific IgM, IgA and IgG and disease severity in unvaccinated infected individuals. In addition to strong IgG1 and IgG3 response, patients with severe disease develop a robust IgG2 and IgG4 response. A comparison of the ratio of IgG1 and IgG3 to IgG2 and IgG4 showed that disease progression is associated with a smaller ratio in both the initial wave of WT and the vaccine breakthrough Delta infections. Time-course analysis revealed that smaller (IgG1 and IgG3)/(IgG2 and IgG4) ratio is associated with disease progression, while the reverse associates with clinical recovery.DiscussionWhile each IgG subclass is associated with disease severity, the balance within the four IgG subclasses may affect disease outcome. Acute disease progression or infection resolution is associated with a specific immunological phenotype that is conserved in both the initial wave of WT and the vaccine breakthrough Delta infections.

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