Regeneration of industrial sugarcane using in-vitro plant apical meristem

Abstract

A rapid micro-propagation of sugarcane, Saccharum officinarum variety NCS 008 using apical meristem shoots were cultured on MS medium supplement with different concentration of BAP (0.1-0.4mg/l) and NAA (0.01-0.04mg/l) for shoot proliferation and elongation. The study showed that highest number of regenerated shoots were obtained from MS medium supplemented with BAP (0.3mg/l) and NAA (0.03mg/l)giving an average of 8 shoot per meristem shoot cultured. Roots were induced using MS medium supplemented with different combinations of NAA and IBA. NAA (3.0mg/l) gave highest rooting percentage of 94.74. The meristem shoot tip demonstrated good response to plant regeneration of sugarcane variety NCS 008 through in-vitro culture.

Keywords

• bap
• culture
• iba
• meristem shoots
• ms
• naa
• sugarcane
• proliferation