Chd1 protects genome integrity at promoters to sustain hypertranscription in embryonic stem cells

Aydan Bulut-Karslioglu; Hu Jin; Yun-Kyo Kim; Brandon Cho; Marcela Guzman-Ayala; Andrew J. K. Williamson; Miroslav Hejna; Maximilian Stötzel; Anthony D. Whetton; Jun S. Song; Miguel Ramalho-Santos

doi:10.1038/s41467-021-25088-3

Nature Communications (Aug 2021)

Chd1 protects genome integrity at promoters to sustain hypertranscription in embryonic stem cells

Aydan Bulut-Karslioglu,
Hu Jin,
Yun-Kyo Kim,
Brandon Cho,
Marcela Guzman-Ayala,
Andrew J. K. Williamson,
Miroslav Hejna,
Maximilian Stötzel,
Anthony D. Whetton,
Jun S. Song,
Miguel Ramalho-Santos

Affiliations

Aydan Bulut-Karslioglu: Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences and Diabetes Center, University of California, San Francisco
Hu Jin: Carl R. Woese Institute for Genomic Biology
Yun-Kyo Kim: Lunenfeld-Tanenbaum Research Institute and Department of Molecular Genetics, University of Toronto
Brandon Cho: Lunenfeld-Tanenbaum Research Institute and Department of Molecular Genetics, University of Toronto
Marcela Guzman-Ayala: Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences and Diabetes Center, University of California, San Francisco
Andrew J. K. Williamson: Stoller Biomarker Discovery Centre, The University of Manchester
Miroslav Hejna: Carl R. Woese Institute for Genomic Biology
Maximilian Stötzel: Max Planck Institute for Molecular Genetics
Anthony D. Whetton: Stoller Biomarker Discovery Centre, The University of Manchester
Jun S. Song: Carl R. Woese Institute for Genomic Biology
Miguel Ramalho-Santos: Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences and Diabetes Center, University of California, San Francisco

DOI: https://doi.org/10.1038/s41467-021-25088-3
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 11

Abstract

Read online

The chromatin remodeler Chd1 mediates hypertranscription in embryonic stem (ES) cells and has been shown to associate with genes transcribed by RNA Polymerase (Pol) I and II. Here the authors provide mechanistic insights into this process and reveal that Chd1 is involved in protecting genome integrity at promoter regions by preventing DNA break accumulation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal