Von Willebrand Factor Collagen-Binding Activity and Von Willebrand Factor-Mediated Platelet Adhesion in Patients with Coronary Artery Disease
Zufar Gabbasov,
Sergey Okhota,
Yuliya Avtaeva,
Olga Saburova,
Ivan Melnikov,
Valentina Shtelmakh,
Sergey Bazanovich,
Konstantin Guria,
Sergey Kozlov
Affiliations
Zufar Gabbasov
Laboratory of Cell Hemostasis, Chazov National Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 121552 Moscow, Russia
Sergey Okhota
Department of Problems of Atherosclerosis, Chazov National Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 121552 Moscow, Russia
Yuliya Avtaeva
Laboratory of Cell Hemostasis, Chazov National Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 121552 Moscow, Russia
Olga Saburova
Laboratory of Cell Hemostasis, Chazov National Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 121552 Moscow, Russia
Ivan Melnikov
Laboratory of Cell Hemostasis, Chazov National Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 121552 Moscow, Russia
Valentina Shtelmakh
Department of Problems of Atherosclerosis, Chazov National Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 121552 Moscow, Russia
Sergey Bazanovich
Laboratory of Cell Hemostasis, Chazov National Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 121552 Moscow, Russia
Konstantin Guria
Laboratory of Cell Hemostasis, Chazov National Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 121552 Moscow, Russia
Sergey Kozlov
Department of Problems of Atherosclerosis, Chazov National Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 121552 Moscow, Russia
In this study, we investigated von Willebrand factor (VWF)-related parameters in 30 patients with stable coronary artery disease (CAD) and 50 patients without CAD. In both groups, the following were measured: the VWF antigen level (VWF:Ag); the VWF ristocetin cofactor activity (VWF:RCo); the VWF collagen-binding activity (VWF:CB); and VWF-mediated platelet adhesion. Platelet adhesion was measured in whole blood at a shear rate of 1300 s−1 using a microfluidic chamber with a collagen-coated surface. VWF:Ag and VWF:RCo were found to be the same in both groups of patients. However, VWF:CB was found to be lower in patients with CAD compared with patients without CAD, with values of 106.7% (82.1; 131.6) and 160.4% (112.5; 218.1), respectively (p p p = 0.011) per 1% increase in VWF:CB activity, and 1.06 (95% CI, 1.03–1.09; p < 0.001) per 1% decrease in GPIb-mediated platelet adhesion. The findings presented in this paper indicate a possible critical role played by complex VWF–collagen-platelet interactions in the development of CAD.