The combined influence of a metabolite complex of Lactobacillus rhamnosus GG and Saccharomyces boulardii with amikacin on infected wounds in models in vivo

Abstract

The aim of the work – to study in vivo the effectiveness of the simultaneous and alternate use of the Lactobacillus rhamnosus GG and Saccharomyces boulardii metabolite complex with an antibacterial preparation in a guinea pig model of skin wound infected with a multiresistant Pseudomonas aruginosa strain to validate prospects for its application in the development of add-on preparations to antibiotic therapy. Materials and methods. The metabolite complex of lactobacteria and saccharomycetes was obtained by culturing cells of probiotic microorganisms in their own ultrasonic disintegrates (MLS). The following were applied to wounds infected with the multiresistant strain of P. aeruginosa twice daily: 0.9 % sodium chloride solution (control group, К), amikacin – AB (group L I), simultaneously AB with MLS (group L II), alternately AB and MLS (group L III). The dynamics of planimetric indicators of wounds (sizing, calculation of the healing area, healing rate, healing rate coefficient, reparative effect) was carried out on days 1, 5, 8 and 11. Antimicrobial activity was evaluated by bacteriological examination of a material from wound samples with identification and determination of the number of colony forming units (CFU) of the pathogen. Results. The combined anti-pseudomonas activity of amikacin and the L. rhamnosus GG and S. boulardii metabolite complex was established. Decreased CFU of P. aeruginosa and acceleration of reparative processes in wounds were observed in groups L I, L II, L III compared to K on days 5 and 8 (P < 0.05). The greater effectiveness of the first proposed alternate method of using AB and MLS was proved in contrast with their simultaneous application. The wound surface areas in L III group were smaller relative to L II (1.8 and 5.0 times, P = 0.03), and L I (2.8 and 9.0 times, P = 0.04) on days 5 and 8, respectively. The increase in synergistic antimicrobial activity due to the new approach of alternate effect was probably due to an increase in antibiotic susceptibility of the test cultures. Conclusions. The presented in the work results of the pronounced synergistic effect of combined using amikacin and the metabolite complex of Lactobacillus rhamnosus GG and Saccharomyces boulardii confirm its promise for practical medicine and the pharmaceutical industry. The data obtained open the potential for developing add-on preparations to antibiotic therapy based on metabolite complexes for the treatment of infected wounds.

Keywords

• metabolites
• antibiotics
• multiresistant bacteria
• infected wounds
• synergistic activity