Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible <i>Hsp70Bb</i> promoter in <i>Drosophila melanogaster</i>

Abstract

AbstractHeat-shock-inducible expression of genes through the use of heat-inducible promoters is commonly used in research despite leaky expression of downstream genes of interest without targeted induction (i.e. heat shock). The development of non-leaky inducible expression systems is of broad interest for both basic and applied studies, to precisely control gene expression. Here we characterize the use of Polycomb response elements and the inducible Heat-shock protein 70BbDrosophila melanogasterCas9Heat-shock protein 70BbCas9Cas9Cas9