Differentiation of primate primordial germ cell-like cells following transplantation into the adult gonadal niche

Enrique Sosa; Di Chen; Ernesto J. Rojas; Jon D. Hennebold; Karen A. Peters; Zhuang Wu; Truong N. Lam; Jennifer M. Mitchell; Meena Sukhwani; Ramesh C. Tailor; Marvin L. Meistrich; Kyle E. Orwig; Gunapala Shetty; Amander T. Clark

doi:10.1038/s41467-018-07740-7

Nature Communications (Dec 2018)

Differentiation of primate primordial germ cell-like cells following transplantation into the adult gonadal niche

Enrique Sosa,
Di Chen,
Ernesto J. Rojas,
Jon D. Hennebold,
Karen A. Peters,
Zhuang Wu,
Truong N. Lam,
Jennifer M. Mitchell,
Meena Sukhwani,
Ramesh C. Tailor,
Marvin L. Meistrich,
Kyle E. Orwig,
Gunapala Shetty,
Amander T. Clark

Affiliations

Enrique Sosa: Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles
Di Chen: Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles
Ernesto J. Rojas: Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles
Jon D. Hennebold: Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center
Karen A. Peters: Department of Obstetrics, Gynecology and Reproductive Sciences and Magee Women’s Research Institute, University of Pittsburgh School of Medicine
Zhuang Wu: Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center
Truong N. Lam: Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center
Jennifer M. Mitchell: Department of Veterinary Medicine and Surgery, University of Texas MD Anderson Cancer Center
Meena Sukhwani: Department of Obstetrics, Gynecology and Reproductive Sciences and Magee Women’s Research Institute, University of Pittsburgh School of Medicine
Ramesh C. Tailor: Department of Radiation Physics, University of Texas MD Anderson Cancer Center
Marvin L. Meistrich: Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center
Kyle E. Orwig: Department of Obstetrics, Gynecology and Reproductive Sciences and Magee Women’s Research Institute, University of Pittsburgh School of Medicine
Gunapala Shetty: Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center
Amander T. Clark: Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles

DOI: https://doi.org/10.1038/s41467-018-07740-7
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 13

Abstract

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Human embryonic stem cells can be differentiated in vitro into primordial germ cell-like cells (PGCLCs) that resemble early primordial germ cells (PGCs). Here the authors transplant PGCLCs generated from rhesus macaque iPSCs into mouse and rhesus macaque seminiferous tubules, which matures these into late PGCs and spermatogonia-like cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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