PERSISTENCE OF MYCOPLASMA DURING UROLITHIASIS

Abstract

Aim. Study the frequency of detection of mycoplasma and ureaplasma in clinical material from urolithiasis patients. Materials and methods. Clinical material samples (blood sera, urine, uroliths) from 31 urolithiasis patients were obtained during operations of urolith removal. Cultural method, LAR and PCR were used in the study. Results. The study of clinical material from 31 patients by PCR has shown, that in 25 individuals (80.6%) DNA of mycoplasma and ureaplasma was detected, and mycoplasma DNA was more frequently detected in uroliths and less - in blood sera. Mycoplasma hominis DNA was detected in clinical material of a significantly larger number of patients. 23 cultures were isolated from 8 patients by a cultural method, that were identified by PCR as M. hominis. All the isolates have grown as «mini-colonies». Even after multiple passages in agar medium, reversion of «mini-colonies» into colonies with a classic morphology was not obtained. Conclusion. A high frequency of detection of mycoplasma and ureaplasma in clinical material of patients with urolithiasis was established. The isolated M. hominis cultures have only grown as «mini-colonies». The phenomenon discovered could give evidence on high variability of mycoplasma and a possibility of existence of previously unknown form of their persistence in human organism.

Keywords

• mycoplasma
• persistence
• pcr
• uroliths