Optimizing the extraction technology of antioxidant substances from Daldinia concentrica by orthogonal experiments

Abstract

This work aimed to optimize the extraction technology of antioxidant substances from Daldinia concentrica by orthogonal experiments. Using ultrasonic assisted extraction process, the effects of ethanol concentration, solid-liquid ratio, and extraction time on scavenging rate of DPPH radical were inspected. According to the result of single-factor experiment and the orthogonal design method, a L16(4 3 ) orthogonal table was used to study the ultrasonic extraction technology of antioxidant active components from the Daldinia concentrica. Based on the analysis on the significance of each level and their interactions, the optimum extraction conditions were as follows: extraction time was 30 min, ethanol concentration was 100% and solid-liquid ratio was 3:100 (concentration was 30 mg⋅mL-1 ). Under these conditions, the DPPH antioxidant activity of the extract was the highest (76.17%). The extraction process could provide theoretical and a practical reference for the development of antioxidant substances in Daldinia concentrica.

Keywords

• antioxidant activity
• daldinia concentrica
• extraction technology
• orthogonal test