VALIDASI METODE ANALISA PENETAPAN KADAR EPIGALOKATEKIN GALAT DENGAN KLT DENSITOMETRI

Abstract

TLC Densitometry is one of method which was used to measure amount of active substance. Active substance of epigallocathechin gallate is one of in green tea extract cream. Method of analysis must be validated to prove that method will give the data close with the real value. Aim of this research is to prove that TLC Densitometry method have liniearity, precision and accuration that fulfill the requirement. This study used concentration 600, 1800 and 3000 ug/ml with 3 replications to measure precision (based on value of CV) and accuration (based on value of recovery). Liniearity was known based on value of r of curve regression between concentration and wide area of chromatogram. LOD and LOQ was calculated based on curve regression. Research showed that method of analyse had liniearity with r= 0,98. Value of CV concentration of 600, 1800 and 3000 ug/ml were 8,18%; 3,19% and 1,53%, respectively and recovery were 88,10%; 99,65% dan 111,33%, respectively. Value of LOD was 827,01 µg/ml and LOQ was 2756,69 µg/ml.

Keywords

• epigallocathechin gallate
• validation
• KLT Densitometry method