Frontiers in Immunology (Aug 2019)

Human Intestinal Enteroids Model MHC-II in the Gut Epithelium

  • Jonathan E. Wosen,
  • Alexandra Ilstad-Minnihan,
  • Julia Y. Co,
  • Wei Jiang,
  • Dhriti Mukhopadhyay,
  • Nielsen Q. Fernandez-Becker,
  • Calvin J. Kuo,
  • Manuel R. Amieva,
  • Manuel R. Amieva,
  • Elizabeth D. Mellins

DOI
https://doi.org/10.3389/fimmu.2019.01970
Journal volume & issue
Vol. 10

Abstract

Read online

The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, “mini-guts” derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs. Our approach enables human enteroids to be used for novel in vitro studies into IEC MHC-II regulation and function during health and disease.

Keywords